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Enhancement of immunity and disease resistance in the white shrimp, Litopenaeus vannamei, by the probiotic, Bacillus subtilis E20.

  • 2009-02
  • Fish & Shellfish Immunology 26(2)
    • D. Tseng
    • P. Ho
    • Sung-Yan Huang
    • Sheng-Chi Cheng
    • Ya‐Li Shiu
    • Chiu-Shia Chiu
    • Chun‐Hung Liu

Abstract

Effects of Bacillus subtilis E20 isolated from fermented soybean on immune parameters and the disease resistance of the white shrimp (Litopenaeus vannamei) after 98 days of B. subtilis E20 feeding were evaluated in this study. Shrimp fed B. subtilis E20-containing diets at concentrations of 10(6) (E206), 10(7) (E207), and 10(8) (E208)cfu kg(-1), respectively, had significantly increased survival rates of 13.3%, 16.7%, and 20%, compared to the control (fed no probiotic) after being challenged with Vibrio alginolyticus. There were no significant differences in the total hemocyte count, respiratory burst, or superoxide dismutase glutathione peroxidase among all treatments. Shrimp fed a higher concentration of the probiotic (E208) exhibited significant increases in phenoloxidase activity, phagocytic activity, and clearance efficiency compared to control shrimp. In addition, B. subtilis E20 showed a weaker inhibitory effect against the growth of Aeromona hydrophila with around a 0.3-cm inhibitory zone, but showed no inhibitory effects against other selected pathogens, such as white shrimp pathogens: V. alginolyticus and Vibrio vulnificus. These results suggest that the increased resistance of shrimp after B. subtilis E20 consumption occurs through immune modifications, such as increases in phenoloxidase activity, phagocytic activity, and clearance efficiency against V. alginolyticus.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Bacillus SubtilisEnhanced Phenoloxidase ActivityBeneficial
Moderate
Bacillus SubtilisImproved Clearance EfficiencyBeneficial
Moderate
Bacillus SubtilisIncreased PMN Cell Phagocytic CapacityBeneficial
Moderate
Bacillus subtilis AB22Enhanced Phenoloxidase ActivityBeneficial
Moderate
Bacillus subtilis AB22Improved Cell Survival RatesBeneficial
Large
Bacillus subtilis AB22Improved Clearance EfficiencyBeneficial
Moderate
Bacillus subtilis AB22Increased PMN Cell Phagocytic CapacityBeneficial
Moderate
Bacillus subtilis DE11Enhanced Phenoloxidase ActivityBeneficial
Moderate
Bacillus subtilis DE11Improved Cell Survival RatesBeneficial
Large
Bacillus subtilis DE11Improved Clearance EfficiencyBeneficial
Moderate
Bacillus subtilis DE11Increased PMN Cell Phagocytic CapacityBeneficial
Moderate
Bacillus subtilis DE111Improved Cell Survival RatesBeneficial
Large
Bacillus subtilis DE111Improved Immune ResponseBeneficial
Moderate
Bacillus subtilis MB40Enhanced Phenoloxidase ActivityBeneficial
Moderate
Bacillus subtilis MB40Improved Clearance EfficiencyBeneficial
Moderate
Bacillus subtilis MB40Increased PMN Cell Phagocytic CapacityBeneficial
Moderate
Bacillus subtilis SNZ-1972Enhanced Phenoloxidase ActivityBeneficial
Moderate
Bacillus subtilis SNZ-1972Improved Clearance EfficiencyBeneficial
Moderate
Bacillus subtilis SNZ-1972Increased PMN Cell Phagocytic CapacityBeneficial
Moderate
Bacillus subtilis SNZ-1972Increased Survival RateBeneficial
Large

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