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Abstract

Gastrointestinal (GI) infection is one of the most common types of infectious diseases. Application of probiotic strains in the control of such infections represents a promising approach. In this study, Lactobacillus fermentum strain 4-17 was isolated from kashkineh, an Iranian cereal fermented food, and identified by sequencing its 16S rRNA gene using universal primers. Its probiotic features, including resistance to acid, bile tolerance, antibacterial activity, resistance to intestinal and gastric juices, and hydrophobicity were evaluated. The ability of this strain to adhere to human intestinal cells as well as the anti-adhesive effect of L. fermentum strain 4-17 against E. coli isolated from patients with urinary tract infection was investigated. L. fermentum strain 4-17 was capable of surviving at various conditions such as low pH values, bile salts exposure, and GI tract environment. It showed 43% cell hydrophobicity. The adhesion level of L. fermentum strain 4-17 to human colon adenocarcinoma Caco-2 cells was 8.5% which was also confirmed by scanning electron microscopy (SEM). Furthermore, this strain showed appropriate anti-adhesive properties (including competition, inhibition and replacement properties) against human pathogenic bacteria. These data suggest that L. fermentum strain 4-17 could be examined further for its useful effects and introduced as a novel candidate probiotic to control GI infection disease.

Keywords: Adhesion; Anti-infection assay; Intestinal epithelial cells; Probiotic.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus fermentumEnhanced Cell Membrane HydrophobicityBeneficial
Moderate
Lactobacillus fermentumImproved Intestinal Cell AdhesionBeneficial
Small
Lactobacillus fermentumReduced Pathogen AdhesionBeneficial
Large
Lactobacillus fermentum HA-179Reduced Adhesion of Escherichia coliBeneficial
Moderate
Lactobacillus fermentum Lf-33Improved Colon Cell AdhesionNeutral
Small
Lactobacillus fermentum Lf-33Increased Survival in Adverse Gastrointestinal ConditionsBeneficial
Moderate
Lactobacillus fermentum Lf-33Reduced Adhesion of Escherichia coliBeneficial
Moderate
Lactobacillus fermentum Lf-33Reduced Cellular HydrophobicityNeutral
Small
Lactobacillus fermentum UALf-32Improved Survival in Gastrointestinal ConditionsBeneficial
Large
Lactobacillus fermentum UALf-32Increased Anti-Adhesive Properties Against Pathogenic BacteriaBeneficial
Large
Lactobacillus fermentum UALf-32Increased Cell Membrane HydrophobicityBeneficial
Moderate
Lactobacillus fermentum UALf-32Reduced Intestinal Cell AdhesionBeneficial
Moderate

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