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Evaluation of in vitro and in vivo potential of Bacillus subtilis MBTDCMFRI Ba37 as a candidate probiont in fish health management.

  • 2021-03
  • Microbial Pathogenesis 152
    • Anusree V. Nair
    • M. Leo Antony
    • N. K. Praveen
    • P. Sayooj
    • T. Raja Swaminathan
    • K. K. Vijayan

Abstract

Bacillus subtilis MBTDCMFRI Ba37 (B. subtilis Ba37), an antibacterial strain isolated from the tropical estuarine habitats of Cochin, was evaluated for in vitro and in vivo potential, and its application as a candidate probiont in fish health management. B. subtilis Ba37 was characterized using their morphological and biochemical properties. It exhibited exoenzymatic activities, tolerance to various physiological conditions and a wide spectrum of antibacterial activity against aquaculture pathogens such as Vibrio and Aeromonas. In co-culture assay, B. subtilis Ba37 inhibited Vibrio anguillarum O1 (V. anguillarum O1) even with the initial cell count of 104 CFUmL-1. Cytotoxicity assay performed using the cell free supernatant (CFS) of B. subtilis Ba37 revealed its non toxic nature. A twenty one days of feeding trial was conducted in juveniles of Etroplus suratensis (E.suratensis) by administrating B. subtilis Ba37 to evaluate its efficacy on growth, immune parameters and antioxidant enzyme activities. Overall the supplementation of B. subtilis Ba37 enhanced significantly (P < 0.05) the survival rate, weight gain, specific growth (SGR), feed conversion ratio (FCR), protein efficiency ratio (PER), and feed efficiency (FE) of the fed animals as compared with the control. The immune parameters and antioxidant activities such as total protein, alkaline phosphatase (ALP), superoxide dismutase (SOD) and catalase were also improved significantly (P < 0.05) while serum alanine aminotransferase (SGOT) and serum aspartate aminotransferase (SGPT) activities were decreased slightly than the control. After fifteen days of challenge test, the fish fed with B. subtilis Ba37 showed higher relative percentage survival (RPS) than the control. Thus the study indicated the advantages of B. subtilis Ba37 to be used as a candidate probiont, which could be effectively utilized in managing diseases in aquaculture systems and to improve the health of the host.

Keywords: Antibacterial activity; Bacillus subtilis Ba37; E.suratensis; Growth performance; Immune response; V. anguillarum.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Bacillus SubtilisEnhanced Antioxidant Enzyme ActivityBeneficial
Large
Bacillus SubtilisEnhanced Protein Efficiency RatioBeneficial
Large
Bacillus SubtilisImproved Feed Conversion RatioBeneficial
Large
Bacillus SubtilisImproved Feed EfficiencyBeneficial
Large
Bacillus SubtilisImproved Immune FunctionBeneficial
Large
Bacillus SubtilisImproved Specific Growth RateBeneficial
Large
Bacillus SubtilisImproved Survival RateBeneficial
Large
Bacillus SubtilisImproved Weight GainBeneficial
Large
Bacillus SubtilisIncreased Relative Survival PercentageBeneficial
Large
Bacillus SubtilisReduced AST LevelsBeneficial
Moderate
Bacillus SubtilisReduced GPT LevelBeneficial
Moderate
Bacillus subtilis MB40Enhanced ImmunityBeneficial
Large
Bacillus subtilis MB40Enhanced Protein Efficiency RatioBeneficial
Large
Bacillus subtilis MB40Improved Feed Conversion RatioBeneficial
Large
Bacillus subtilis MB40Improved Feed EfficiencyBeneficial
Large
Bacillus subtilis MB40Improved Specific Growth RateBeneficial
Large
Bacillus subtilis MB40Improved Survival RateBeneficial
Large
Bacillus subtilis MB40Increased Body WeightBeneficial
Large
Bacillus subtilis MB40Increased Relative Survival PercentageBeneficial
Large
Bacillus subtilis SNZ-1972Decreased Serum Alanine Aminotransferase (SGOT) LevelsBeneficial
Moderate
Bacillus subtilis SNZ-1972Enhanced Protein Efficiency RatioBeneficial
Large
Bacillus subtilis SNZ-1972Improved Feed Conversion RatioBeneficial
Large
Bacillus subtilis SNZ-1972Improved Feed EfficiencyBeneficial
Large
Bacillus subtilis SNZ-1972Improved Immune FunctionBeneficial
Large
Bacillus subtilis SNZ-1972Improved Specific Growth RateBeneficial
Large
Bacillus subtilis SNZ-1972Improved Survival RateBeneficial
Large
Bacillus subtilis SNZ-1972Increased Body WeightBeneficial
Large
Bacillus subtilis SNZ-1972Increased Relative Survival PercentageBeneficial
Large
Bacillus subtilis SNZ-1972Reduced Serum Aspartate Aminotransferase (SGPT)Beneficial
Moderate

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