Skip to main content
Supplement Research and Comparison WebsiteBest Price Guarantee
Supplement Research and Comparison Website

Study Design

Population
LPS-treated RAW264.7 cells and DSS-induced colitis mice
Methods
70% ethanol extract and water extract preparation, UHPLC-Q-Orbitrap-MS analysis, cell experiments, and DSS-induced colitis mouse model
Funding
Unclear
During 'Fengtang' plum fruit processing, the seeds as by-products are discarded as waste. Nevertheless, its seed has edible and medicinal value. Thus, we investigated the phytochemical constituents of 'Fengtang' plum seed and explored its anti-colitis effects and related mechanisms. 'Fengtang' plum seed 70% ethanol extract (EE) and water extract (WE) were found to be abundant in phenolic and flavonoid compounds. Fifty-five phytochemicals identified by UHPLC-Q-Orbitrap-MS analysis included 12 phenols and 8 flavonoids. In LPS-treated RAW264.7 cells, EE inhibited the ROS/MAPK/NF-κB pathway, thereby repressing IL-6, IL-1β, NO, PGE2, and TNF-α oversecretion. Additionally, the DSS-induced pathological symptoms of colon mice, including weight loss, colon shortening, and DAI score rise, were considerably alleviated. It also significantly suppressed inflammatory cytokine secretion and reduced ROS generation by increasing CAT and SOD activities and decreasing MDA levels in tissue fluid and serum. Therefore, 'Fengtang' plum seed has potential utilization value in the field of functional foods.

Research Insights

SupplementDoseHealth OutcomeEffect TypeEffect SizeSource
Back to top