Skip to main content

Abstract

We investigated the mechanism by which an Indian indigenous probiotic culture, Lactobacillus rhamnosus S1K3, could overcome the pathogenic strain Salmonella enterica with an emphasis on the response at the intestinal mucosal level after long-term (30days) consumption. S1K3 was able to produce antimicrobial compounds against the pathogens. The probiotic adhered strongly to intestinal epithelium and maintained its integrity in presence of Salmonella through stimulation of tight junction and antimicrobial peptide genes in vitro. Mice prefed for 30days with S1K3-fermented milk exhibited low incidence of pathogenic Salmonella at mucosal and systemic levels. The probiotic induced TLRs transcripts at the Peyer's patches, followed by an increase in the Secretory-IgA in intestinal fluid, the IgA-secreting cells in lamina propria of small intestine and the IgA level in serum. Moreover, S1K3 maintained the protein level of IL-12, increased the IL-4 and reduced the TGF-β level in intestinal fluid/serum at the later stage of infection. All these actions concurred to lower the count of Salmonella in feces, its invasion in spleen, liver and intestine tissues and improved the health status of probiotic-fed group. In view of this performance, S1K3 appears to be a suitable candidate for the development of nutraceutical food.

Keywords: Intestine; Lactobacillus; Mucosal immunity; Probiotic; Salmonella.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus rhamnosus MAK79L08RImproved Cytokine LevelsBeneficial
Moderate
Lactobacillus rhamnosus MAK79L08RImproved Intestinal Barrier IntegrityBeneficial
Moderate
Lactobacillus rhamnosus MAK79L08RIncreased Fecal Secretory IgA LevelsBeneficial
Moderate
Lactobacillus rhamnosus MAK79L08RReduced Salmonella IncidenceBeneficial
Moderate
Lactobacillus rhamnosus R0343NDImproved Immune ResponseBeneficial
Large
Lactobacillus rhamnosus R0343NDReduced Salmonella IncidenceBeneficial
Moderate
Lactobacillus rhamnosus UALr-06Improved Intestinal Mucosal ImmunityBeneficial
Large
Lactobacillus rhamnosus UALr-06Reduced Salmonella Enterica InvasionBeneficial
Large
Lactobacillus rhamnosus UALr-06Regulated Cytokine LevelsBeneficial
Moderate
Lactobacillus rhamnosus UALr-18Enhanced Tight Junction IntegrityBeneficial
Moderate
Lactobacillus rhamnosus UALr-18Improved Overall Health StatusBeneficial
Large
Lactobacillus rhamnosus UALr-18Increased Secretory IgA LevelBeneficial
Large
Lactobacillus rhamnosus UALr-18Reduced Salmonella IncidenceBeneficial
Moderate
Lactobacillus rhamnosus UALrh-18Enhanced Immune ResponseBeneficial
Moderate
Lactobacillus rhamnosus UALrh-18Enhanced Intestinal IntegrityBeneficial
Large
Lactobacillus rhamnosus UALrh-18Improved Cytokine LevelsBeneficial
Moderate
Lactobacillus rhamnosus UALrh-18Reduced Pathogen InfectionBeneficial
Large

Pillser helps you make informed health decisions by providing comprehensive, unbiased information about supplements. This includes detailed research on supplement ingredients, their benefits, potential risks, and their overall efficacy. You can contribute by sharing your feedback and suggestions.

Send us an email at support@pillser.com.