Ganoderma lucidum dietary fiber and lactobacillus fermentum CECT5716 alleviate constipation via regulation of gut barrier and microbiota.

2025-12
International journal of biological macromolecules 333
- Caihong Wang
- Qianyang Zhang
- Chaozhi Wei
- Yuanxiang Jin

PubMed: 41232882
DOI: 10.1016/j.ijbiomac.2025.149010

Study Design

Population: mice
Methods: investigated the effect of Ganoderma lucidum dietary fiber (GLDF) and Lactobacillus fermentum CECT5716 on ameliorating constipation induced by loperamide (LOP)

Animal Study

The rising incidence of constipation, as well as the side effects of constipation medications, have prompted people to look for substances of natural origin to treat constipation. In this study, we investigated the effect of Ganoderma lucidum dietary fiber (GLDF) and Lactobacillus fermentum CECT5716 on ameliorating constipation induced by loperamide (LOP) and elucidated the underlying mechanism. The results showed that GLDF and L. fermentum CECT5716 alleviated constipation symptoms, evidenced by enhanced fecal water content, alleviated difficult defecation, and improved intestinal propulsion. The combination treatment also inhibited inflammatory factors in the colon, restored the impaired intestinal tight junction, and suppressed colonic aquaporin expression. Moreover, the GLDF and L. fermentum CECT5716 complex proficiently regulates gut microbiota while augmenting the generation of short-chain fatty acids (SCFAs)-with acetic acid, propionic acid, and butyric acid as the primary elevated metabolites. Our findings suggest that GLDF and L. fermentum CECT5716 mitigated constipation in mice, highlighting their potential as a combined functional food.

Research Insights

Lactobacillus fermentum→Improved Constipation
L. fermentum CECT5716 alleviated constipation symptoms, evidenced by enhanced fecal water content, alleviated difficult defecation, and improved intestinal propulsion.
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum→Improved Intestinal Barrier Function
restored the impaired intestinal tight junction, and suppressed colonic aquaporin expression.
Effect
Beneficial
Effect size
Small
Lactobacillus fermentum→Increased Short-Chain Fatty Acid Production
augmenting the generation of short-chain fatty acids (SCFAs)-with acetic acid, propionic acid, and butyric acid as the primary elevated metabolites.
Effect
Beneficial
Effect size
Small
Lactobacillus fermentum→Reduced Colonic Inflammation
The combination treatment also inhibited inflammatory factors in the colon
Effect
Beneficial
Effect size
Small
Lactobacillus fermentum CECT5716→Improved Constipation
L. fermentum CECT5716 alleviated constipation symptoms, evidenced by enhanced fecal water content, alleviated difficult defecation, and improved intestinal propulsion.
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum CECT5716→Improved Intestinal Barrier Function
restored the impaired intestinal tight junction, and suppressed colonic aquaporin expression.
Effect
Beneficial
Effect size
Small
Lactobacillus fermentum CECT5716→Reduced Colonic Inflammation
The combination treatment also inhibited inflammatory factors in the colon
Effect
Beneficial
Effect size
Small