Skip to main content
Supplement Research and Comparison WebsiteBest Price Guarantee
Supplement Research and Comparison Website

Genomic and Phylogenetic Analysis of Lactiplantibacillus plantarum L125, and Evaluation of Its Anti-Proliferative and Cytotoxic Activity in Cancer Cells

  • 2021-11-19
  • Biomedicines 9(11)
    • Konstantinos Tegopoulos
    • Odysseas Sotirios Stergiou
    • D. Kiousi
    • Margaritis Tsifintaris
    • Ellie Koletsou
    • A. Papageorgiou
    • A. Argyri
    • Nikos G Chorianopoulos
    • A. Galanis
    • P. Kolovos

Abstract

Lactiplantibacillus plantarum is a diverse species that includes nomadic strains isolated from a variety of environmental niches. Several L. plantarum strains are being incorporated in fermented foodstuffs as starter cultures, while some of them have also been characterized as probiotics. In this study, we present the draft genome sequence of L. plantarum L125, a potential probiotic strain presenting biotechnological interest, originally isolated from a traditional fermented meat product. Phylogenetic and comparative genomic analysis with other potential probiotic L. plantarum strains were performed to determine its evolutionary relationships. Furthermore, we located genes involved in the probiotic phenotype by whole genome annotation. Indeed, genes coding for proteins mediating host-microbe interactions and bile salt, heat and cold stress tolerance were identified. Concerning the potential health-promoting attributes of the novel strain, we determined that L. plantarum L125 carries an incomplete plantaricin gene cluster, in agreement with previous in vitro findings, where no bacteriocin-like activity was detected. Moreover, we showed that cell-free culture supernatant (CFCS) of L. plantarum L125 exerts anti-proliferative, anti-clonogenic and anti-migration activity against the human colon adenocarcinoma cell line, HT-29. Conclusively, L. plantarum L125 presents desirable probiotic traits. Future studies will elucidate further its biological and health-related properties.

Keywords: Lactiplantibacillus plantarum; anti-proliferative activity; comparative genomics; genomics; phylogenetic analysis; probiotics; whole-genome sequencing.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactiplantibacillus plantarumReduced Cancer Cell ProliferationBeneficial
Moderate
Lactiplantibacillus plantarum KABP™-022Reduced Cancer Cell ProliferationBeneficial
Moderate
Back to top