Genomic mechanisms underlying the adaptation of Anaerobutyricum species to the human host.

2025-11
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 135

PubMed: 41109491
DOI: 10.1016/j.meegid.2025.105844

Study Design

Population: 84 Anaerobutyricum genomes
Methods: comprehensive genomic analysis of 84 Anaerobutyricum genomes, based on the average nucleotide identity (ANI), average amino acid identity (AAI) and core genes phylogenetic tree analysis

The Anaerobutyricum genus, comprising Anaerobutyricum hallii and Anaerobutyricum soehngenii, is prevalent in the human gut microbiota and produces short-chain fatty acids (SCFAs) that influence host metabolism and inflammation. However, systematic genomic studies on this genus are limited. In this study, we conducted a comprehensive genomic analysis of 84 Anaerobutyricum genomes, based on the average nucleotide identity (ANI), average amino acid identity (AAI) and core genes phylogenetic tree analysis, we clustered the Anaerobutyricum genus to Anaerobutyricum spp., Anaerobutyricum hallii and Anaerobutyricum soehngenii. Specifically, Anaerobutyricum hallii and Anaerobutyricum soehngenii are specific to human hosts, possessing longer genomes and lower GC content. They contain more integrative and conjugative elements (ICEs), which genetically encoded dltA, mbtB and dhbE genes that responds for the biosynthesis of siderophore group nonribosomal peptides, which might contribute to its colonization. In addition, ICEs in Anaerobutyricum hallii and Anaerobutyricum soehngenii could also pose a risk for antibiotic resistance gene transfer. Our study advances the understanding the of Anaerobutyricum's and host adaptability, highlighting the importance of genomic analysis in refining taxonomic boundaries and assessing the safety of using these bacteria as probiotics.

Research Insights

Supplement	Dose	Health Outcome	Effect Type	Effect Size	Source
Anaerobutyricum hallii	—	Not Reported	Neutral	Small	View source The abstract describes genomic analysis of Anaerobutyricum species and their host adaptation, but does not report any intervention or observed health outcomes from consuming Anaerobutyricum hallii.