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Genotoxicity and subchronic general toxicity assessments of Lactobacillus curvatus WiKim 38 using Sprague-Dawley rats.

  • 2021-06
  • Food and Chemical Toxicology 152
    • Ji-Seok Han
    • Seng-Min Back
    • Jae-woo Cho
    • Heejin Park
    • Woojin Kim
    • S. Park
    • Jung-Ho Noh
    • Yongbum Kim
    • Byoung-Seok Lee


Lactobacillus curvatus WiKim 38 (LCW), isolated from kimchi, has shown novel immunomodulatory and anti-inflammatory properties. In the present study, to obtain data on the safety of LCW, we performed three genotoxicity (bacterial reverse mutation, chromosome aberration, and micronucleus) and two general toxicity (single-dosing and 13-week repeated-dosing) studies. In the genotoxicity assessment, LCW showed no increased reverse mutation for 4 strains of Salmonella typhimurium and a strain of Escherichia coli. In addition, LCW did not induce chromosome aberrations at concentrations up to 5000 μg/mL in cultured Chinese hamster lung (CHL) cells and did not induce an increased frequency of micronuclei in the bone marrow cells of rats at concentrations up to 2000 mg/kg. In the acute toxicity study using Sprague-Dawley (SD) rats, the approximate lethal dose of LCW was determined to be over 5000 mg/kg body weight (b.w.) in both sexes. Finally, in the subchronic toxicity study, no LCW-related adverse effects were observed at concentrations up to 5000 mg/kg b.w./day. Consequently, LCW is considered not to have mutagenic effects, and its no-observed-adverse-effect-level (NOAEL) is 5000 mg/kg b.w., equivalent to approximately 4.71 × 109 CFU/kg b.w., suggesting the LCW could be a potential probiotic for humans based on its safety profile.

Keywords: Genotoxicity; Lactobacillus curvatus WiKim 38; Probiotics; Safety assessment; Subchronic general toxicity.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus curvatusAbsence of Acute ToxicityNeutral
Lactobacillus curvatusNo Increase in Reverse MutationNeutral
Lactobacillus curvatusNo Induced Chromosomal AberrationsNeutral
Lactobacillus curvatusNo Subchronic ToxicityNeutral

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