Goat-derived Lactobacillus amylovorus improved floppy kid syndrome via regulating gut microflora.
- 2025-08-19
- BMC veterinary research 21(1)
- Cheng Cheng
- Yan Zheng
- Xin Wang
- Jianping Tao
- Darong Cheng
- PubMed: 40830792
- DOI: 10.1186/s12917-025-04967-7
Background
Floppy Kid Syndrome (FKS) severely restricts goat farming due to high mortality from metabolic disturbances and gut dysbiosis. Here, we aimed to isolate a goat-derived probiotic and evaluate its capacity to restore gut homeostasis in FKSaffected goats.Results
Lactobacillus amylovorus isolates was obtained via selective colony morphology, Gramstain and catalase testing, and confirmed by 16 S rRNA sequencing (≥ 98% identity). The isolate survived at pH 2.0 (72%) and pH 3.0 (85%), tolerated 0.3% bile salts (survival > 10%), and retained > 40% viability after 60 s at 60 °C. In disk diffusion assays, inhibition zones averaged 16 mm against Escherichia coli and 12 mm against Staphylococcus aureus. In FKSaffected goats receiving 1 × 108 CFU/day for 3 days, Shannon diversity increased by 25% (p < 0.05), Simpson index by 18% (p < 0.05), and shared species with healthy controls rose from 8 to 17. Relative abundance of EscherichiaShigella declined by 40%, while Lactobacillus and Ruminococcus increased by 30% and 22%, respectively (p < 0.05).Conclusions
Goat-derived L. amylovorus displays robust acid, bile and heat tolerance, inhibits key pathogens, and effectively regulates gut microbiota in FKSaffected goats. These findings support its promise as a novel probiotic intervention for preventing and treating Floppy Kid Syndrome.Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus amylovorus | Improved Gut Microbiota Balance | Beneficial | Moderate |