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Gut microbiota analysis reveals a marked shift to bifidobacteria by a starter infant formula containing a synbiotic of bovine milk-derived oligosaccharides and Bifidobacterium animalis subsp. lactis CNCM I-3446.

  • 2016-01-18
  • Environmental Microbiology 18(7)
    • U. Simeoni
    • B. Berger
    • Jana Junick
    • M. Blaut
    • S. Pecquet
    • E. Rezzonico
    • D. Grathwohl
    • N. Sprenger
    • H. Brüssow
    • H. Szajewska
    • J. Bartoli
    • V. Brévaut-Malaty
    • M. Borszewska-Kornacka
    • W. Feleszko
    • P. François
    • C. Gire
    • M. Leclaire
    • J-M Maurin
    • S. Schmidt
    • A. Skórka
    • C. Squizzaro
    • J. Verdot
    • Study Team

Abstract

Non-digestible milk oligosaccharides were proposed as receptor decoys for pathogens and as nutrients for beneficial gut commensals like bifidobacteria. Bovine milk contains oligosaccharides, some of which are structurally identical or similar to those found in human milk. In a controlled, randomized double-blinded clinical trial we tested the effect of feeding a formula supplemented with a mixture of bovine milk-derived oligosaccharides (BMOS) generated from whey permeate, containing galacto-oligosaccharides and 3'- and 6'-sialyllactose, and the probiotic Bifidobacterium animalis subsp. lactis (B. lactis) strain CNCM I-3446. Breastfed infants served as reference group. Compared with a non-supplemented control formula, the test formula showed a similar tolerability and supported a similar growth in healthy newborns followed for 12 weeks. The control, but not the test group, differed from the breast-fed reference group by a higher faecal pH and a significantly higher diversity of the faecal microbiota. In the test group the probiotic B. lactis increased by 100-fold in the stool and was detected in all supplemented infants. BMOS stimulated a marked shift to a bifidobacterium-dominated faecal microbiota via increases in endogenous bifidobacteria (B. longum, B. breve, B. bifidum, B. pseudocatenulatum).

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Bifidobacterium animalisBalanced Gut MicrobiotaBeneficial
Moderate
Bifidobacterium animalisEnhanced Growth and DevelopmentNeutral
Moderate
Bifidobacterium animalisIncreased Bifidobacterium LevelsBeneficial
Large
Bifidobacterium animalisReduced Faecal pHBeneficial
Moderate
Bifidobacterium animalis subsp. lactisEnhanced Gut Microbiota CompositionBeneficial
Large
Bifidobacterium animalis subsp. lactisIncreased Bifidobacteria LevelsBeneficial
Large
Bifidobacterium animalis subsp. lactisSimilar Growth Rate to Breastfed InfantsNeutral
Moderate
Bifidobacterium animalis subsp. lactis BLC1Comparable TolerabilityNeutral
Moderate
Bifidobacterium animalis subsp. lactis BLC1Increased Beneficial Gut BacteriaBeneficial
Large
Bifidobacterium animalis subsp. lactis BLC1Increased Fecal Acidity Similar to Breastfed InfantsBeneficial
Moderate
Bifidobacterium animalis subsp. lactis BS-01Improved Bifidobacterium-Dominated MicrobiotaBeneficial
Large
Bifidobacterium animalis subsp. lactis BS-01Increased Probiotic LevelsBeneficial
Large
Bifidobacterium animalis subsp. lactis BS-01Normalized Stool pH Comparable to Breastfed InfantsBeneficial
Moderate
Bifidobacterium animalis subsp. lactis CECT 8145Improved Growth in InfantsNeutral
Large
Bifidobacterium animalis subsp. lactis CECT 8145Increased Bifidobacterium AbundanceBeneficial
Large
Bifidobacterium animalis subsp. lactis CECT 8145Increased Probiotic LevelsBeneficial
Large
Bifidobacterium animalis subsp. lactis CECT 8145Reduced Faecal pHBeneficial
Moderate
Bifidobacterium animalis subsp. lactis HN019Enhanced Probiotic PresenceBeneficial
Large
Bifidobacterium animalis subsp. lactis HN019Improved Gut HealthBeneficial
Moderate
Bifidobacterium animalis subsp. lactis HN019Increased Bifidobacteria LevelsBeneficial
Large
Bifidobacterium animalis subsp. lactis LAFTI B94Comparable Growth with Breastfed InfantsBeneficial
Moderate
Bifidobacterium animalis subsp. lactis LAFTI B94Increased Levels of BifidobacteriaBeneficial
Large
Bifidobacterium animalis subsp. lactis VK2Improved Growth in InfantsNeutral
Large
Bifidobacterium animalis subsp. lactis VK2Increased Bifidobacterium Levels in the GutBeneficial
Large
Bifidobacterium animalis subsp. lactis VK2Increased Probiotic Concentration in StoolBeneficial
Large
Bifidobacterium animalis subsp. lactis VK2Stable Fecal pHBeneficial
Moderate
Bifidobacterium bifidumIncreased Bifidobacteria in Gut MicrobiotaBeneficial
Large
Bifidobacterium bifidumReduced Faecal pHBeneficial
Moderate
Bifidobacterium bifidumStool Colonization with Probiotic Bifidobacterium lactisBeneficial
Large
Bifidobacterium bifidum BB-06Improved Gut HealthBeneficial
Moderate
Bifidobacterium bifidum BB-06Increased Bifidobacteria LevelsBeneficial
Large
Bifidobacterium bifidum/lactis Bb-02Increased Bifidobacterium PopulationBeneficial
Large
Bifidobacterium bifidum/lactis Bb-02Increased Growth in NewbornsNeutral
Moderate
Bifidobacterium bifidum/lactis Bb-02Stable Fecal pHBeneficial
Moderate
Bifidobacterium lactisImproved Gut Microbiome CompositionBeneficial
Large
Bifidobacterium lactisIncreased Probiotic B. lactis LevelsBeneficial
Large
Bifidobacterium lactisReduced Faecal pHBeneficial
Moderate
Bifidobacterium lactis B420Comparable Growth with Breastfed InfantsBeneficial
Moderate
Bifidobacterium lactis B420Increased Bifidobacterium AbundanceBeneficial
Large
Bifidobacterium lactis B420Increased Presence of Bifidobacterium lactisBeneficial
Large
Bifidobacterium lactis B420Reduced Faecal pHBeneficial
Moderate
Bifidobacterium lactis Bb-02Improved Bifidobacterium-Dominated MicrobiotaBeneficial
Large
Bifidobacterium lactis Bb-02Increased Fecal Bifidobacterium lactis LevelsBeneficial
Moderate
Bifidobacterium lactis Bb-02Reduced Faecal pHBeneficial
Moderate
Bifidobacterium lactis Bb-02Reduced Fecal Microbiota DiversityNeutral
Moderate
Bifidobacterium lactis Bi-07Increased Gut Colonization by Beneficial BifidobacteriaBeneficial
Large
Bifidobacterium lactis Bi-07Reduced Fecal Microbiota DiversityNeutral
Moderate
Bifidobacterium lactis Bi-07Sustained Healthy GrowthNeutral
Moderate
Bifidobacterium lactis BL818Improved Gut Microbiome CompositionBeneficial
Large
Bifidobacterium lactis BL818Increased Bifidobacteria CountBeneficial
Large
Bifidobacterium lactis BLA80Improved Gut Health (Lower Faecal pH)Beneficial
Moderate
Bifidobacterium lactis BLA80Improved Gut Microbiota CompositionBeneficial
Large
Bifidobacterium lactis BLA80Increased Bifidobacterium ConcentrationBeneficial
Large
Bifidobacterium lactis BLA80Maintained Growth RateBeneficial
Small
Bifidobacterium lactis BLC-1Comparable Growth with Breastfed InfantsBeneficial
Moderate
Bifidobacterium lactis BLC-1Improved Bifidobacterium-Dominated MicrobiotaBeneficial
Large
Bifidobacterium lactis BLC-1Increased Fecal Bifidobacterium lactis LevelsBeneficial
Moderate
Bifidobacterium lactis BS01Increased Bifidobacteria LevelsBeneficial
Large
Bifidobacterium lactis BS01Reduced Faecal pHBeneficial
Moderate
Bifidobacterium lactis BS01Stool Colonization with Probiotic Bifidobacterium lactisBeneficial
Large
Bifidobacterium lactis HA-194Increased Bifidobacteria in Gut MicrobiotaBeneficial
Large
Bifidobacterium lactis HDS Bb-02Increased Bifidobacterium Dominance in Faecal MicrobiotaBeneficial
Large
Bifidobacterium lactis HDS Bb-02Reduced Faecal pHBeneficial
Moderate
Bifidobacterium lactis ISS BifIncreased Bifidobacterium Animalis in StoolBeneficial
Large
Bifidobacterium lactis ISS BifIncreased Bifidobacterium-Dominated Gut MicrobiotaBeneficial
Large
Bifidobacterium lactis/longumImproved Gut Health (Lower Faecal pH)Beneficial
Moderate
Bifidobacterium lactis/longumIncreased Bifidobacterium-Dominated Gut MicrobiotaBeneficial
Large
Bifidobacterium lactis/longumIncreased Fecal Recovery of Bifidobacterium Animalis Subsp LactisBeneficial
Large
Bifidobacterium lactis/longumNo Change in Newborn GrowthNeutral
Small
Bifidobacterium lactis MAK16B42LIncreased Bifidobacterium Dominance in Faecal MicrobiotaBeneficial
Large
Bifidobacterium lactis MAK16B42LIncreased Gut Bifidobacterium LevelsBeneficial
Large
Bifidobacterium lactis MAK16B42LReduced Faecal pHBeneficial
Moderate
Bifidobacterium lactis R101-8Comparable Growth with Breastfed InfantsBeneficial
Moderate
Bifidobacterium lactis R101-8Improved Gut Microbiome CompositionBeneficial
Large
Bifidobacterium lactis R101-8Increased Fecal Bifidobacterium lactis LevelsBeneficial
Moderate
Bifidobacterium lactis R101-8Reduced Faecal pHBeneficial
Moderate
Bifidobacterium lactis SD-5219Improved Bifidobacterium-Dominated MicrobiotaBeneficial
Large
Bifidobacterium lactis SD-5219Improved Gut Microbiota CompositionBeneficial
Large
Bifidobacterium lactis SD-5219Reduced Faecal pHBeneficial
Moderate
Bifidobacterium lactis VK2Reduced Fecal Microbiota DiversityNeutral
Moderate

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