Agromyces excoecariae sp. nov., isolated from the rhizosphere soil of mangrove Excoecaria agallocha.
- 2026-01-14
- International journal of systematic and evolutionary microbiology 76(1)
- Yuan Li
- Yiyang Zhang
- Jinyao Song
- Yueting Zhou
- Fang Liu
- Yan Huang
- PubMed: 41532985
- DOI: 10.1099/ijsem.0.007026
Study Design
- Methods
- Isolation from rhizosphere soil of Excoecaria agallocha, phylogenetic analysis based on 16S rRNA gene sequences, ANI and dDDH comparisons, chemotaxonomic characterization including polar lipids, fatty acids, menaquinones, cell-wall amino acids, and genome analysis.
- Funding
- Unclear
A novel Agromyces strain, designated as M3QZ16-3T, was isolated from the rhizosphere soil of Excoecaria agallocha, collected from the Maowei Sea Mangrove Nature Reserve in the Guangxi Zhuang Autonomous Region, China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M3QZ16-3T was closely related to species of the genus Agromyces and shared the highest similarity of 99.26% with Agromyces kandeliae Q22T. The average nucleotide identity and digital DNA-DNA hybridization values between strain M3QZ16-3T and the type strain of A. kandeliae Q22T were 88.2% and 34.4%, respectively. The polar lipids of strain M3QZ16-3T comprised diphosphatidylglycerol, phosphatidylglycerol, one unidentified glycolipid, one unidentified polar lipid and two unidentified lipids. The major cellular fatty acids were anteiso-C15:0, anteiso-C17:0, iso-C15:0 and iso-C16:0, and the predominant menaquinones (MK; vitamin K₂) were MK-12 (78.3%) and MK-11 (14.3%). Cell-wall amino acids were 2,4-diaminobutyric acid, glutamic acid, glycine and alanine. The genome size of strain M3QZ16-3T was 3.8 Mb, with a DNA G+C content value of 71.7 mol%. Based on the above descriptions, we propose strain M3QZ16-3T to represent a novel species of the genus Agromyces, for which the name Agromyces excoecariae sp. nov. is proposed. The type strain is M3QZ16-3T (=MCCC 1K07179T=JCM 34940T).