Bifidobacterium adolescentis Strengthens Gut Barrier in Post-Voyage Functional Constipation.

2025-12-17
International journal of molecular sciences 26(24)

PubMed: 41465567
DOI: 10.3390/ijms262412142

Study Design

Population: C57BL/6J mice
Methods: C57BL/6J mice received fecal suspension treatment following a 12-day navigation period, which was subsequently followed by a 12-day oral administration of B. adolescentis.

Rigorous Journal

Prolonged periods of sailing may contribute to the development of functional constipation, which can significantly impair an individual's work efficiency. Currently, the efficacy of Bifidobacteria in treating functional constipation is gaining recognition. However, since the therapeutic effects of Bifidobacteria are strain-specific, further research is required on strains isolated from pre-voyage fecal samples. This study examines the role of gut microbiota in post-stroke constipation, aiming to identify specific microbial biomarkers for the development of targeted therapeutic strategies. B. adolescentis was identified through metagenomic analysis and subsequently isolated for validation. In the experimental group (EG), C57BL/6J mice received fecal suspension treatment following a 12-day navigation period, which was subsequently followed by a 12-day oral administration of B. adolescentis. After treatment, EG significantly improved fecal volume, intestinal motility, and goblet cells; reversed microbial ecological imbalance; reduced pathogens (E. coli and Klebsiella) by restoring arginine/bile acid metabolism, decreasing Tauro-ursodeoxycholic acid (TUDCA) content, 5-Hydroxytryptamine 4 Receptor (5-HT4R)/Slc8a1 signaling, and Ca²⁺ signaling pathway; and restoring beneficial species (B. adolescentis, Pseudomonas aeruginosa). This study provides new insights into probiotics in improving human intestinal health.

Research Insights

Supplement	Dose	Health Outcome	Effect Type	Effect Size	Source
Bifidobacterium adolescentis	—	Improved Constipation	Beneficial	Moderate	View source EG significantly improved fecal volume, intestinal motility, and goblet cells; reversed microbial ecological imbalance; reduced pathogens
Bifidobacterium adolescentis	—	Improved Gut Barrier Function	Beneficial	Moderate	View source Bifidobacterium adolescentis Strengthens Gut Barrier ... significantly improved fecal volume, intestinal motility, and goblet cells
Bifidobacterium adolescentis	—	Improved Gut Microbiota Balance	Beneficial	Moderate	View source reversed microbial ecological imbalance; reduced pathogens (E. coli and Klebsiella)