Immune enhancing effects of Lactobacillus acidophilus LAFTI L10 and Lactobacillus paracasei LAFTI L26 in mice.
- 2007-04-01
- International Journal of Food Microbiology 115(1)
- Gunaranjan Paturi
- M. Phillips
- Mark R. Jones
- K. Kailasapathy
- PubMed: 17134782
- DOI: 10.1016/J.IJFOODMICRO.2006.10.007
Study Design
- Type
- Clinical Trial
- Population
- BALB/c mice
- Methods
- In vivo experiment with mice.
- Animal Study
Abstract
The immune enhancing properties of Lactobacillus acidophilus LAFTI L10 and Lactobacillus paracasei LAFTI L26 in mice were investigated. Each mouse (BALB/c) was orally fed with cultures of either L. acidophilus or L. paracasei at 10(8) CFU/50 mul per day for 14 days. The effect of these strains on immunoglobulin A, interleukin-10 and interferon gamma producing cells in the gut immune system was determined by immunofluorescence assays. Systemic immune responses were analysed in mice serum upon euthanising after a 14-day feeding trial to estimate cytokines such as IL-10 and IFN-gamma by enzyme-linked immunosorbent assays. L. acidophilus and L. paracasei strains demonstrated an increase in the number of IgA producing cells, IL-10 and IFN-gamma cytokine producing cells in the small intestine. In the systemic immune response, mice fed with L. acidophilus or L. paracasei also enhanced the secretion of anti-inflammatory cytokine (IL-10) and pro-inflammatory cytokine (IFN-gamma). The results of this study suggest that L. acidophilus and L. paracasei were able to enhance specific gut and systemic immune responses in mice.