Impact of Bifidobacterium animalis subsp. lactis BB-12 and, Lactobacillus acidophilus LA-5-containing yoghurt, on fecal bacterial counts of healthy adults.
- 2011-09
- International Journal of Food Microbiology 149(1)
- P. Savard
- B. Lamarche
- Marie-Ève Paradis
- H. Thiboutot
- E. Laurin
- D. Roy
- PubMed: 21296446
- DOI: 10.1016/j.ijfoodmicro.2010.12.026
Abstract
This randomized, placebo-controlled, double blind, parallel dose-response study investigated the impact of 4-week commercial yoghurt consumption supplemented with Bifidobacterium animalis subsp. lactis (BB-12) and Lactobacillus acidophilus (LA-5) on fecal bacterial counts of healthy adults. Fifty-eight volunteers were randomly assigned to three different groups: 1. placebo (no probiotic, no starter and no green tea extract); 2. Yoptimal (10(9)cfu/100g of BB-12 and LA-5 and 40mg of green tea extract) and 3. Yoptimal-10 (10(10)cfu/100g of BB-12, 10(9)cfu/100g of LA-5 and 40mg of green tea extract). These yoghurt products also contained Lactobacillus delbrueckii subsp. bulgaricus (10(7)cfu/100g) and Streptococcus thermophilus (10(10)cfu/100g). The quantitative PCR (qPCR) results showed that there were significant increases (P=0.02) in bifidobacteria counts with the Yoptimal treatment as compared to baseline. The fecal numbers of B. animalis subsp. lactis and LA-5 significantly increased in the two probiotic treatments compared to the placebo treatment. Viable counts of fecal lactobacilli were significantly higher (P=0.05) and those of enterococci were significantly lower (P=0.04) after the intervention when compared to placebo. No significant difference was observed between treatments in volunteers' weight, waist girth, blood pressure, fasting plasma triglyceride and HDL-C concentrations, as well as cholesterol/HDL-cholesterol ratio. However, a significant increase in plasma cholesterol levels was observed in the placebo group (P=0.0018) but the levels remained stable in the two probiotic yoghurt groups. These results show that probiotic strains supplemented in the form of yoghurt remain active during gut transit and are associated with an increase in beneficial bacteria and a reduction in potentially pathogenic bacteria. This trial was registered at clinicaltrials.gov as NCT00730626.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Bifidobacterium animalis | Increased Fecal Lactobacilli | Beneficial | Small |
| Bifidobacterium animalis | Increased Gut Bifidobacteria | Beneficial | Moderate |
| Bifidobacterium animalis | Stable Plasma Cholesterol Levels | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis | Reduced Enterococci Counts | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis B420 | Increased Beneficial Bacterial Counts | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis B420 | Increased Viable Fecal Lactobacilli Count | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis B420 | Reduced Fecal Enterococci Levels | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis B420 | Stable Plasma Cholesterol Levels | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis BB-12 | Increased Gut Bifidobacteria | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BLC1 | Increased Fecal Lactobacilli | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis BLC1 | Increased Gut Bifidobacteria | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BLC1 | Reduced Enterococci Levels | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BLC1 | Stable Plasma Cholesterol Levels | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis BS-01 | Increased Fecal Bifidobacteria | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BS-01 | Increased Fecal Lactobacilli Viability | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BS-01 | Reduced Enterococci Counts | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BS-01 | Stable Plasma Cholesterol Levels | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis CECT 8145 | Increased Fecal Bifidobacteria Levels | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis CECT 8145 | Increased Fecal Lactobacilli | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis CECT 8145 | Reduced Fecal Enterococci Levels | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis CECT 8145 | Stable Plasma Cholesterol Levels | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis HN019 | Increased Fecal Lactobacilli | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis HN019 | Increased Gut Bifidobacteria | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis HN019 | Reduced Fecal Enterococci Levels | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis HN019 | Stable Plasma Cholesterol Levels | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis LAFTI B94 | Increased Fecal Bifidobacteria Levels | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis LAFTI B94 | Increased Fecal Lactobacilli | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis LAFTI B94 | Reduced Enterococci Levels | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis LAFTI B94 | Stable Plasma Cholesterol Levels | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis VK2 | Increased Fecal Lactobacilli | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis VK2 | Increased Gut Bifidobacteria | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis VK2 | Increased Probiotic B. lactis Levels | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis VK2 | Reduced Fecal Enterococci Levels | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis VK2 | Stable Plasma Cholesterol Levels | Beneficial | Small |
| Bifidobacterium bifidum BB-06 | Increased Fecal Lactobacilli | Beneficial | Small |
| Bifidobacterium bifidum BB-06 | Increased Gut Bifidobacteria | Beneficial | Moderate |
| Bifidobacterium bifidum BB-06 | Reduced Fecal Enterococci Levels | Beneficial | Small |
| Bifidobacterium bifidum BB-06 | Stable Plasma Cholesterol Levels | Beneficial | Small |
| Bifidobacterium bifidum/lactis Bb-02 | Increased Bifidobacteria Count | Beneficial | Moderate |
| Bifidobacterium bifidum/lactis Bb-02 | Increased Fecal Lactobacilli Viability | Beneficial | Moderate |
| Bifidobacterium bifidum/lactis Bb-02 | Reduced Enterococci Counts | Beneficial | Moderate |
| Bifidobacterium lactis | Increased Beneficial Gut Bacteria | Beneficial | Moderate |
| Bifidobacterium lactis | Increased Fecal Lactobacillus Count | Beneficial | Moderate |
| Bifidobacterium lactis | Increased Specific Probiotic Strains | Beneficial | Moderate |
| Bifidobacterium lactis | Stable Plasma Cholesterol Levels | Beneficial | Small |
| Bifidobacterium lactis Bb-02 | Increased Fecal Lactobacilli | Beneficial | Small |
| Bifidobacterium lactis Bb-02 | Increased Gut Bifidobacteria | Beneficial | Moderate |
| Bifidobacterium lactis Bb-02 | Increased Probiotic B. lactis Levels | Beneficial | Moderate |
| Bifidobacterium lactis Bb-02 | Reduced Fecal Enterococci Levels | Beneficial | Small |
| Bifidobacterium lactis Bb-02 | Stable Plasma Cholesterol Levels | Beneficial | Small |
| Bifidobacterium lactis BB-12 | Increased Bifidobacteria Count | Beneficial | Moderate |
| Bifidobacterium lactis BB-12 | Increased Fecal Lactobacilli Viability | Beneficial | Moderate |
| Bifidobacterium lactis BB-12 | Increased Fecal Recovery of Bifidobacterium Animalis Subsp Lactis | Beneficial | Moderate |
| Bifidobacterium lactis BB-12 | Reduced Enterococci Counts | Beneficial | Moderate |
| Bifidobacterium lactis BB-12 | Stable Plasma Cholesterol Levels | Beneficial | Small |
| Bifidobacterium lactis BL818 | Increased Fecal Lactobacilli | Beneficial | Small |
| Bifidobacterium lactis BL818 | Increased Gut Bifidobacteria | Beneficial | Moderate |
| Bifidobacterium lactis BL818 | Reduced Enterococci Counts | Beneficial | Moderate |
| Bifidobacterium lactis BL818 | Stable Plasma Cholesterol Levels | Beneficial | Small |
| Bifidobacterium lactis BLA80 | Increased Fecal Lactobacilli | Beneficial | Small |
| Bifidobacterium lactis BLA80 | Increased Gut Bifidobacteria | Beneficial | Moderate |
| Bifidobacterium lactis BLA80 | Reduced Enterococci Counts | Beneficial | Moderate |
| Bifidobacterium lactis BLA80 | Stable Plasma Cholesterol Levels | Beneficial | Small |
| Bifidobacterium lactis BS01 | Increased Beneficial Bacteria (Bifidobacteria) | Beneficial | Moderate |
| Bifidobacterium lactis BS01 | Increased Fecal Recovery of Bifidobacterium Animalis Subsp Lactis | Beneficial | Moderate |
| Bifidobacterium lactis BS01 | Increased Lactobacillus Counts | Beneficial | Moderate |
| Bifidobacterium lactis BS01 | Reduced Enterococci Counts | Beneficial | Moderate |
| Bifidobacterium lactis BS01 | Stable Plasma Cholesterol Levels | Beneficial | Small |
| Bifidobacterium lactis ISS Bif | Increased Gut Bifidobacteria | Beneficial | Moderate |
| Bifidobacterium lactis ISS Bif | Increased Viable Fecal Lactobacilli Count | Beneficial | Moderate |
| Bifidobacterium lactis ISS Bif | Reduced Fecal Enterococci Levels | Beneficial | Small |
| Bifidobacterium lactis ISS Bif | Stable Plasma Cholesterol Levels | Beneficial | Small |
| Bifidobacterium lactis/longum | Increased Gut Bifidobacteria | Beneficial | Moderate |
| Bifidobacterium lactis/longum | Increased Viable Lactobacillus Counts | Beneficial | Moderate |
| Bifidobacterium lactis/longum | Reduced Potentially Pathogenic Bacteria | Beneficial | Moderate |
| Bifidobacterium lactis/longum | Stable Plasma Cholesterol Levels | Beneficial | Small |
| Bifidobacterium lactis MAK16B42L | Increased Fecal Lactobacilli | Beneficial | Small |
| Bifidobacterium lactis MAK16B42L | Increased Fecal Probiotic Strains | Beneficial | Moderate |
| Bifidobacterium lactis MAK16B42L | Increased Gut Bifidobacteria | Beneficial | Moderate |
| Bifidobacterium lactis MAK16B42L | Reduced Fecal Enterococci Levels | Beneficial | Small |
| Bifidobacterium lactis MAK16B42L | Stable Plasma Cholesterol Levels | Beneficial | Small |
| Bifidobacterium lactis R101-8 | Increased Fecal Lactobacilli | Beneficial | Small |
| Bifidobacterium lactis R101-8 | Increased Gut Bifidobacteria | Beneficial | Moderate |
| Bifidobacterium lactis R101-8 | Reduced Fecal Enterococci Levels | Beneficial | Small |
| Bifidobacterium lactis R101-8 | Stable Plasma Cholesterol Levels | Beneficial | Small |
| Bifidobacterium lactis SD-5219 | Increased Bifidobacteria Count | Beneficial | Moderate |
| Bifidobacterium lactis SD-5219 | Increased Fecal Lactobacilli Viability | Beneficial | Moderate |
| Bifidobacterium lactis SD-5219 | Reduced Fecal Enterococci Levels | Beneficial | Small |
| Bifidobacterium lactis SD-5219 | Stable Plasma Cholesterol Levels | Beneficial | Small |
| Bifidobacterium lactis UABla-12 | Increased Bifidobacteria Count | Beneficial | Moderate |
| Bifidobacterium lactis UABla-12 | Increased Lactobacillus Counts | Beneficial | Moderate |
| Bifidobacterium lactis UABla-12 | Reduced Enterococci Counts | Beneficial | Moderate |
| Bifidobacterium lactis UABla-12 | Stable Plasma Cholesterol Levels | Beneficial | Small |
| Lactobacillus acidophilus LA1 | Increased Fecal Lactobacilli | Beneficial | Small |
| Lactobacillus acidophilus LA1 | Reduced Fecal Enterococci Levels | Beneficial | Small |
| Lactobacillus acidophilus LA1 | Stable Plasma Cholesterol Levels | Beneficial | Small |
| Lactobacillus acidophilus LA-5 | Improved Fecal Microbiota Composition | Beneficial | Moderate |
| Lactobacillus acidophilus LA-5 | Increased Fecal Lactobacilli | Beneficial | Small |
| Lactobacillus acidophilus LA-5 | Reduced Fecal Enterococci | Beneficial | Moderate |
| Lactobacillus acidophilus LA-5 | Stable Plasma Cholesterol Levels | Beneficial | Small |
| Lactobacillus acidophilus LA85 | Increased Fecal Lactobacilli | Beneficial | Small |
| Lactobacillus acidophilus LA85 | Increased Gut Bifidobacteria | Beneficial | Moderate |
| Lactobacillus acidophilus LA85 | Reduced Enterococci Counts | Beneficial | Moderate |
| Lactobacillus acidophilus LA85 | Stable Plasma Cholesterol Levels | Beneficial | Small |