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Impact of Bifidobacterium animalis subsp. lactis BB-12 and, Lactobacillus acidophilus LA-5-containing yoghurt, on fecal bacterial counts of healthy adults.

  • 2011-09
  • International Journal of Food Microbiology 149(1)
    • P. Savard
    • B. Lamarche
    • Marie-Ève Paradis
    • H. Thiboutot
    • E. Laurin
    • D. Roy

Abstract

This randomized, placebo-controlled, double blind, parallel dose-response study investigated the impact of 4-week commercial yoghurt consumption supplemented with Bifidobacterium animalis subsp. lactis (BB-12) and Lactobacillus acidophilus (LA-5) on fecal bacterial counts of healthy adults. Fifty-eight volunteers were randomly assigned to three different groups: 1. placebo (no probiotic, no starter and no green tea extract); 2. Yoptimal (10(9)cfu/100g of BB-12 and LA-5 and 40mg of green tea extract) and 3. Yoptimal-10 (10(10)cfu/100g of BB-12, 10(9)cfu/100g of LA-5 and 40mg of green tea extract). These yoghurt products also contained Lactobacillus delbrueckii subsp. bulgaricus (10(7)cfu/100g) and Streptococcus thermophilus (10(10)cfu/100g). The quantitative PCR (qPCR) results showed that there were significant increases (P=0.02) in bifidobacteria counts with the Yoptimal treatment as compared to baseline. The fecal numbers of B. animalis subsp. lactis and LA-5 significantly increased in the two probiotic treatments compared to the placebo treatment. Viable counts of fecal lactobacilli were significantly higher (P=0.05) and those of enterococci were significantly lower (P=0.04) after the intervention when compared to placebo. No significant difference was observed between treatments in volunteers' weight, waist girth, blood pressure, fasting plasma triglyceride and HDL-C concentrations, as well as cholesterol/HDL-cholesterol ratio. However, a significant increase in plasma cholesterol levels was observed in the placebo group (P=0.0018) but the levels remained stable in the two probiotic yoghurt groups. These results show that probiotic strains supplemented in the form of yoghurt remain active during gut transit and are associated with an increase in beneficial bacteria and a reduction in potentially pathogenic bacteria. This trial was registered at clinicaltrials.gov as NCT00730626.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Bifidobacterium animalisIncreased Fecal LactobacilliBeneficial
Small
Bifidobacterium animalisIncreased Gut BifidobacteriaBeneficial
Moderate
Bifidobacterium animalisStable Plasma Cholesterol LevelsBeneficial
Small
Bifidobacterium animalis subsp. lactisReduced Enterococci CountsBeneficial
Moderate
Bifidobacterium animalis subsp. lactis B420Increased Beneficial Bacterial CountsBeneficial
Moderate
Bifidobacterium animalis subsp. lactis B420Increased Viable Fecal Lactobacilli CountBeneficial
Moderate
Bifidobacterium animalis subsp. lactis B420Reduced Fecal Enterococci LevelsBeneficial
Small
Bifidobacterium animalis subsp. lactis B420Stable Plasma Cholesterol LevelsBeneficial
Small
Bifidobacterium animalis subsp. lactis BB-12Increased Gut BifidobacteriaBeneficial
Moderate
Bifidobacterium animalis subsp. lactis BLC1Increased Fecal LactobacilliBeneficial
Small
Bifidobacterium animalis subsp. lactis BLC1Increased Gut BifidobacteriaBeneficial
Moderate
Bifidobacterium animalis subsp. lactis BLC1Reduced Enterococci LevelsBeneficial
Moderate
Bifidobacterium animalis subsp. lactis BLC1Stable Plasma Cholesterol LevelsBeneficial
Small
Bifidobacterium animalis subsp. lactis BS-01Increased Fecal BifidobacteriaBeneficial
Moderate
Bifidobacterium animalis subsp. lactis BS-01Increased Fecal Lactobacilli ViabilityBeneficial
Moderate
Bifidobacterium animalis subsp. lactis BS-01Reduced Enterococci CountsBeneficial
Moderate
Bifidobacterium animalis subsp. lactis BS-01Stable Plasma Cholesterol LevelsBeneficial
Small
Bifidobacterium animalis subsp. lactis CECT 8145Increased Fecal Bifidobacteria LevelsBeneficial
Moderate
Bifidobacterium animalis subsp. lactis CECT 8145Increased Fecal LactobacilliBeneficial
Small
Bifidobacterium animalis subsp. lactis CECT 8145Reduced Fecal Enterococci LevelsBeneficial
Small
Bifidobacterium animalis subsp. lactis CECT 8145Stable Plasma Cholesterol LevelsBeneficial
Small
Bifidobacterium animalis subsp. lactis HN019Increased Fecal LactobacilliBeneficial
Small
Bifidobacterium animalis subsp. lactis HN019Increased Gut BifidobacteriaBeneficial
Moderate
Bifidobacterium animalis subsp. lactis HN019Reduced Fecal Enterococci LevelsBeneficial
Small
Bifidobacterium animalis subsp. lactis HN019Stable Plasma Cholesterol LevelsBeneficial
Small
Bifidobacterium animalis subsp. lactis LAFTI B94Increased Fecal Bifidobacteria LevelsBeneficial
Moderate
Bifidobacterium animalis subsp. lactis LAFTI B94Increased Fecal LactobacilliBeneficial
Small
Bifidobacterium animalis subsp. lactis LAFTI B94Reduced Enterococci LevelsBeneficial
Moderate
Bifidobacterium animalis subsp. lactis LAFTI B94Stable Plasma Cholesterol LevelsBeneficial
Small
Bifidobacterium animalis subsp. lactis VK2Increased Fecal LactobacilliBeneficial
Small
Bifidobacterium animalis subsp. lactis VK2Increased Gut BifidobacteriaBeneficial
Moderate
Bifidobacterium animalis subsp. lactis VK2Increased Probiotic B. lactis LevelsBeneficial
Moderate
Bifidobacterium animalis subsp. lactis VK2Reduced Fecal Enterococci LevelsBeneficial
Small
Bifidobacterium animalis subsp. lactis VK2Stable Plasma Cholesterol LevelsBeneficial
Small
Bifidobacterium bifidum BB-06Increased Fecal LactobacilliBeneficial
Small
Bifidobacterium bifidum BB-06Increased Gut BifidobacteriaBeneficial
Moderate
Bifidobacterium bifidum BB-06Reduced Fecal Enterococci LevelsBeneficial
Small
Bifidobacterium bifidum BB-06Stable Plasma Cholesterol LevelsBeneficial
Small
Bifidobacterium bifidum/lactis Bb-02Increased Bifidobacteria CountBeneficial
Moderate
Bifidobacterium bifidum/lactis Bb-02Increased Fecal Lactobacilli ViabilityBeneficial
Moderate
Bifidobacterium bifidum/lactis Bb-02Reduced Enterococci CountsBeneficial
Moderate
Bifidobacterium lactisIncreased Beneficial Gut BacteriaBeneficial
Moderate
Bifidobacterium lactisIncreased Fecal Lactobacillus CountBeneficial
Moderate
Bifidobacterium lactisIncreased Specific Probiotic StrainsBeneficial
Moderate
Bifidobacterium lactisStable Plasma Cholesterol LevelsBeneficial
Small
Bifidobacterium lactis Bb-02Increased Fecal LactobacilliBeneficial
Small
Bifidobacterium lactis Bb-02Increased Gut BifidobacteriaBeneficial
Moderate
Bifidobacterium lactis Bb-02Increased Probiotic B. lactis LevelsBeneficial
Moderate
Bifidobacterium lactis Bb-02Reduced Fecal Enterococci LevelsBeneficial
Small
Bifidobacterium lactis Bb-02Stable Plasma Cholesterol LevelsBeneficial
Small
Bifidobacterium lactis BB-12Increased Bifidobacteria CountBeneficial
Moderate
Bifidobacterium lactis BB-12Increased Fecal Lactobacilli ViabilityBeneficial
Moderate
Bifidobacterium lactis BB-12Increased Fecal Recovery of Bifidobacterium Animalis Subsp LactisBeneficial
Moderate
Bifidobacterium lactis BB-12Reduced Enterococci CountsBeneficial
Moderate
Bifidobacterium lactis BB-12Stable Plasma Cholesterol LevelsBeneficial
Small
Bifidobacterium lactis BL818Increased Fecal LactobacilliBeneficial
Small
Bifidobacterium lactis BL818Increased Gut BifidobacteriaBeneficial
Moderate
Bifidobacterium lactis BL818Reduced Enterococci CountsBeneficial
Moderate
Bifidobacterium lactis BL818Stable Plasma Cholesterol LevelsBeneficial
Small
Bifidobacterium lactis BLA80Increased Fecal LactobacilliBeneficial
Small
Bifidobacterium lactis BLA80Increased Gut BifidobacteriaBeneficial
Moderate
Bifidobacterium lactis BLA80Reduced Enterococci CountsBeneficial
Moderate
Bifidobacterium lactis BLA80Stable Plasma Cholesterol LevelsBeneficial
Small
Bifidobacterium lactis BS01Increased Beneficial Bacteria (Bifidobacteria)Beneficial
Moderate
Bifidobacterium lactis BS01Increased Fecal Recovery of Bifidobacterium Animalis Subsp LactisBeneficial
Moderate
Bifidobacterium lactis BS01Increased Lactobacillus CountsBeneficial
Moderate
Bifidobacterium lactis BS01Reduced Enterococci CountsBeneficial
Moderate
Bifidobacterium lactis BS01Stable Plasma Cholesterol LevelsBeneficial
Small
Bifidobacterium lactis ISS BifIncreased Gut BifidobacteriaBeneficial
Moderate
Bifidobacterium lactis ISS BifIncreased Viable Fecal Lactobacilli CountBeneficial
Moderate
Bifidobacterium lactis ISS BifReduced Fecal Enterococci LevelsBeneficial
Small
Bifidobacterium lactis ISS BifStable Plasma Cholesterol LevelsBeneficial
Small
Bifidobacterium lactis/longumIncreased Gut BifidobacteriaBeneficial
Moderate
Bifidobacterium lactis/longumIncreased Viable Lactobacillus CountsBeneficial
Moderate
Bifidobacterium lactis/longumReduced Potentially Pathogenic BacteriaBeneficial
Moderate
Bifidobacterium lactis/longumStable Plasma Cholesterol LevelsBeneficial
Small
Bifidobacterium lactis MAK16B42LIncreased Fecal LactobacilliBeneficial
Small
Bifidobacterium lactis MAK16B42LIncreased Fecal Probiotic StrainsBeneficial
Moderate
Bifidobacterium lactis MAK16B42LIncreased Gut BifidobacteriaBeneficial
Moderate
Bifidobacterium lactis MAK16B42LReduced Fecal Enterococci LevelsBeneficial
Small
Bifidobacterium lactis MAK16B42LStable Plasma Cholesterol LevelsBeneficial
Small
Bifidobacterium lactis R101-8Increased Fecal LactobacilliBeneficial
Small
Bifidobacterium lactis R101-8Increased Gut BifidobacteriaBeneficial
Moderate
Bifidobacterium lactis R101-8Reduced Fecal Enterococci LevelsBeneficial
Small
Bifidobacterium lactis R101-8Stable Plasma Cholesterol LevelsBeneficial
Small
Bifidobacterium lactis SD-5219Increased Bifidobacteria CountBeneficial
Moderate
Bifidobacterium lactis SD-5219Increased Fecal Lactobacilli ViabilityBeneficial
Moderate
Bifidobacterium lactis SD-5219Reduced Fecal Enterococci LevelsBeneficial
Small
Bifidobacterium lactis SD-5219Stable Plasma Cholesterol LevelsBeneficial
Small
Bifidobacterium lactis UABla-12Increased Bifidobacteria CountBeneficial
Moderate
Bifidobacterium lactis UABla-12Increased Lactobacillus CountsBeneficial
Moderate
Bifidobacterium lactis UABla-12Reduced Enterococci CountsBeneficial
Moderate
Bifidobacterium lactis UABla-12Stable Plasma Cholesterol LevelsBeneficial
Small
Lactobacillus acidophilus LA1Increased Fecal LactobacilliBeneficial
Small
Lactobacillus acidophilus LA1Reduced Fecal Enterococci LevelsBeneficial
Small
Lactobacillus acidophilus LA1Stable Plasma Cholesterol LevelsBeneficial
Small
Lactobacillus acidophilus LA-5Improved Fecal Microbiota CompositionBeneficial
Moderate
Lactobacillus acidophilus LA-5Increased Fecal LactobacilliBeneficial
Small
Lactobacillus acidophilus LA-5Reduced Fecal EnterococciBeneficial
Moderate
Lactobacillus acidophilus LA-5Stable Plasma Cholesterol LevelsBeneficial
Small
Lactobacillus acidophilus LA85Increased Fecal LactobacilliBeneficial
Small
Lactobacillus acidophilus LA85Increased Gut BifidobacteriaBeneficial
Moderate
Lactobacillus acidophilus LA85Reduced Enterococci CountsBeneficial
Moderate
Lactobacillus acidophilus LA85Stable Plasma Cholesterol LevelsBeneficial
Small

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