Skip to main content

In vitro and in vivo examination of anticolonization of pathogens by Lactobacillus paracasei FJ861111.1.

  • 2015-10
  • Journal of Dairy Science 98(10)
    • Kan Deng
    • Tingtao Chen
    • Qinglong Wu
    • Hongbo Xin
    • Q. Wei
    • Ping Hu
    • Xiaolei Wang
    • Xin Wang
    • Hua Wei
    • N. Shah

Abstract

Very limited information exists on the exclusion of pathogens by probiotics in the gut of the host challenged with pathogens. In this study, we tested probiotic characteristics in vitro and anticolonization ability of Lactobacillus paracasei FJ861111.1 in mice infected with selected pathogenic microorganisms. The in vitro results indicated that L. paracasei FJ861111.1 had a high survival in acidic conditions at pH 2.5 and bile salt concentration at 0.3%, and strong inhibition ability against common pathogens including Shigella dysenteriae, Staphylococcus aureus, Cronobacter sakazakii, Escherichia coli, and Candida albicans. The cell adhesion assays showed that L. paracasei FJ861111.1 exhibited strong adherence to HT-29 cells and excluded the adhesion of selected food-borne pathogens to HT-29 cells. The in vivo results showed that fermented milk with L. paracasei and viili (a Nordic yogurt product) significantly improved the population of total bacteria and of Lactobacillus in the feces of mice, and significantly inhibited the colonization of C. albicans to the intestines of mice post-C. albicans infection. Thus, it appears that this strain could be used as a probiotic organism for manufacturing functional fermented milk.

Keywords: Lactobacillus paracasei; anticolonization; pathogenic infection; probiotic characteristics.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus paracasei DSM 13434Enhanced Bile Salt ResistanceBeneficial
Moderate
Lactobacillus paracasei DSM 13434Enhanced Intestinal Epithelial Cell AdhesionBeneficial
Large
Lactobacillus paracasei DSM 13434Improved Survival in Acidic ConditionsBeneficial
Moderate
Lactobacillus paracasei DSM 13434Increased Beneficial Gut Bacterial PopulationBeneficial
Large
Lactobacillus paracasei DSM 13434Reduced Candida albicans ColonizationBeneficial
Large
Lactobacillus paracasei DSM 13434Reduced Pathogenic Microorganism ActivityBeneficial
Large
Lactobacillus paracasei HA-196Improved Gut MicrobiotaBeneficial
Moderate
Lactobacillus paracasei HA-196Reduced Pathogen ColonizationBeneficial
Large
Lactobacillus paracasei IMC 502Increased Beneficial Gut BacteriaBeneficial
Moderate
Lactobacillus paracasei IMC 502Intestinal Cell AdherenceBeneficial
Large
Lactobacillus paracasei IMC 502Reduced Intestinal Colonization of Candida albicansBeneficial
Large
Lactobacillus paracasei IMC 502Reduced Pathogen GrowthBeneficial
Large
Lactobacillus paracasei MCC1849Improved Gut MicrobiotaBeneficial
Moderate
Lactobacillus paracasei MCC1849Increased Intestinal Cell AdhesionBeneficial
Large
Lactobacillus paracasei MCC1849Increased Survival in Acidic EnvironmentBeneficial
Large
Lactobacillus paracasei MCC1849Reduced Pathogen ActivityBeneficial
Large
Lactobacillus paracasei MCC1849Reduced Pathogen ColonizationBeneficial
Large
Lactobacillus paracasei UALpc-04Improved Gut Microbiota CompositionBeneficial
Moderate
Lactobacillus paracasei UALpc-04Reduced Colonization of C. albicans in Intestines Post-InfectionBeneficial
Large
Lactobacillus paracasei UALpc-04Reduced Pathogen ActivityBeneficial
Large
Lactobacillus paracasei UALpc-04Strong Adherence of HT-29 CellsBeneficial
Large
Lactobacillus paracasei VPro 224Improved Gut MicrobiotaBeneficial
Moderate
Lactobacillus paracasei VPro 224Reduced Candida albicans ColonizationBeneficial
Large
Lactobacillus paracasei VPro 224Reduced Pathogenic LoadBeneficial
Large

Pillser helps you make informed health decisions by providing comprehensive, unbiased information about supplements. This includes detailed research on supplement ingredients, their benefits, potential risks, and their overall efficacy. You can contribute by sharing your feedback and suggestions.

Send us an email at support@pillser.com.