In vitro assessment of histamine and lactate production by a multi-strain synbiotic.
- 2021-12-02
- Journal of food science and technology 59(9)
- PubMed: 35875231
- DOI: 10.1007/s13197-021-05327-7
Study Design
- Methods
- in vitro production of histamine and d-lactate by a 24-strain daily synbiotic
Recent studies suggest histamine and d-lactate may negatively impact host health. As excess histamine is deleterious to the host, the identification of bacterial producers has contributed to concerns over the consumption of probiotics or live microorganisms in fermented food items. Some probiotic products have been suspected of inducing d-lactic-acidosis; an illness associated with neurocognitive symptoms such as ataxia. The goals of the present study were to test the in vitro production of histamine and d-lactate by a 24-strain daily synbiotic and to outline methods that others can use to test for their production. Using enzymatic based assays, no significant production of histamine was observed compared to controls (P > 0.05), while d-lactate production was comparable to a commercially available probiotic with no associated health risk. These assays provide a means to add to the safety profile of synbiotic and probiotic products.
Research Insights
| Supplement | Dose | Health Outcome | Effect Type | Effect Size | Source |
|---|---|---|---|---|---|
| Bifidobacterium infantis SD-6720 | — | Reduced D-Lactate Production | Neutral | Small | View source"d-lactate production was comparable to a commercially available probiotic with no associated health risk" |
| Bifidobacterium lactis SD-5219 | — | Comparable D-Lactate Production | Neutral | Small | View sourced-lactate production was comparable to a commercially available probiotic with no associated health risk |
| Lactobacillus brevis SD5214 | — | Reduced D-Lactate Production | Neutral | Small | View source"d-lactate production was comparable to a commercially available probiotic with no associated health risk" |
| Lactobacillus casei SD5213 | — | Comparable D-Lactate Production | Neutral | Small | View sourced-lactate production was comparable to a commercially available probiotic with no associated health risk |
| Lactobacillus paracasei SD5275 | — | Reduced D-Lactate Production | Neutral | Small | View source"d-lactate production was comparable to a commercially available probiotic with no associated health risk" |
| Lactobacillus plantarum SD5209 | — | Comparable D-Lactate Production | Neutral | Small | View sourced-lactate production was comparable to a commercially available probiotic with no associated health risk |