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In Vitro Probiotic and Antioxidant Potential of Lactococcus lactis subsp. cremoris LL95 and Its Effect in Mice Behaviour

  • 2019-04-22
  • Nutrients 11(4)
    • Juliana B Ramalho
    • Melina Bucco Soares
    • Cristiano Chiapinotto Spiazzi
    • D. F. Bicca
    • V. M. Soares
    • J. G. Pereira
    • W. P. da Silva
    • C. P. Sehn
    • F. Cibin


The composition of intestinal microbiota is widely believed to not only affect gut health but also influence behaviour. This study aimed to evaluate the probiotic characteristics, antioxidant activity, and antidepressant- and anxiolytic-like activities of Lactococcus lactis subsp. cremoris LL95. This strain showed probiotic properties such as resistance in a simulated gastric tract model and survival at different concentrations of NaCl and bile salts. Moreover, antioxidant activity of LL95 was demonstrated through DPPH radical scavenging activity, scavenging of ABTS•+ radical and ferric ion reducing antioxidant power (FRAP) assays. Female C57BL/6 mice received LL95 orally at a dose of 109 UFC/day for 28 days. LL95 improved depressive- and anxiety-like behaviour, demonstrated by decreased immobility time in the tail suspension test and forced swim test and increased per cent of time spent in the open arms on the elevated plus maze. These findings indicate the potential antioxidant activity of LL95 and its role in behaviour, suggesting that probiotic may have therapeutic applications.

Keywords: Lactococcus lactis subsp. cremoris; antioxidant activity; anxiety; depression; probiotic.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Leuconostoc cremoris V95Improved Antioxidant ActivityBeneficial
Leuconostoc cremoris V95Reduced AnxietyBeneficial
Leuconostoc cremoris V95Reduced Depression SymptomsBeneficial
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