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In vivo antimalarial activity of a probiotic bacterium Lactobacillus sakei isolated from traditionally fermented milk in BALB/c mice infected with Plasmodium berghei ANKA.

  • 2021-11
  • Journal of Ethnopharmacology 280
    • Liliane Laure Toukam
    • Bertrand Tatsinkou Fossi
    • G. S. Taiwé
    • Raymond Bess Bila
    • David Denis Feugaing Sofeu
    • Enyong Peter Ivo
    • E. Achidi


Ethnopharmacological relevance: Milk production, processing and consumption are integral part of traditional practices in Fulani tribe of Cameroon. It has been observed that Fulani are resistant to malaria. Dairy products traditionally processed by Fulani are intensively used in the ritual treatment of malarial, inflammations and behavioural disorders. Many studies have demonstrated that fermented milk is a rich source of probiotic bacteria. However, the antimalarial activity of probiotics isolated from this natural source has not been experimentally tested.

Aim of the study: Hence, this study was therefore aimed at evaluating the antimalarial activity of a probiotic bacterium Lactobacillus sakei isolated from traditionally fermented milk in mice infected with chloroquine sensitive Plasmodium berghei ANKA.

Materials and methods: The probiotic bacterium was isolated from the Cameroonian Mborro Fulani's traditionally fermented milk and identified using the 16S r RNA gene sequencing. The schizontocidal activity of Lactobacillus sakei on established malaria infection was evaluated. Eighty-four healthy young adult Balb/c mice infected with Plasmodium berghei parasite were randomly divided into two sets of seven group of six mice each, and were given three different doses of Lactobacillus sakei, chloroquine and sulfadoxine/pyrimethamine for seven and fourteen days respectively. The level of parasitaemia, body temperature, survival time and haematological parameters were evaluated.

Results: The parasite growth inhibition was observed to increase with increasing dose of probiotic bacterium with maximum suppression being 100 % at dose 3 on day 20. Also, the probiotic bacterium significantly prevented body weight loss and was associated with body temperature reduction and prevented (p<0.05) a decrease in haematological parameters compared to that untreated malaria infected mice.

Conclusion: The results obtained suggest that Lactobacillus sakei is a probiotic bacterium with antimalarial activity in mice infected with chloroquine sensitive Plasmodium berghei.

Keywords: Fermented milk; Fulani; Haematological parameters; Malaria; Parasitaemia; Probiotics.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus sakei proBio65Inhibited Parasite GrowthBeneficial
Lactobacillus sakei proBio65Maintained Body WeightBeneficial
Lactobacillus sakei proBio65Prevention of Haematological Parameter DeclineBeneficial
Lactobacillus sakei proBio65Reduced Body TemperatureBeneficial

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