Insights on the enhanced wound healing potential of russelioside B isolated from Caralluma quadrangula via modulation of AMPK/Beclin-1 and P-62 autophagy pathway.
- 2026-06
- Burns : journal of the International Society for Burn Injuries 52(5)
- PubMed: 41980583
- DOI: 10.1016/j.burns.2026.108008
Study Design
- Sample size
- n = 6
- Population
- Rats (n=6 per group) with full-thickness excisional wound
- Methods
- Random assignment to six groups (normal control, positive control, MEBO-treated, and 0.5%, 1%, 1.5% RB). Topical application once daily for 14 days.
- Duration
- 14 days
- Funding
- Unclear
This study aimed to evaluate the efficacy of russelioside B (RB), isolated from Caralluma quadrangula, in promoting wound healing through the modulation of key autophagy markers. For topical application, RB was incorporated into a sodium carboxymethyl cellulose (NaCMC) gel. Rats were randomly assigned to six groups (n = 6 per group): a normal control group, a positive control (PC) group, an MEBO®-treated group, and three groups treated with RB at concentrations of 0.5%, 1%, and 1.5%. Under anesthesia, a full-thickness excisional wound with a diameter of 1 cm was created on each rat. Treatments were applied topically once daily for 14 days. Tissue samples were collected and assessed for levels of superoxide dismutase (SOD), malondialdehyde (MDA), and nitric oxide (NO). Gene expression of vascular endothelial growth factor (VEGF) and tumor necrosis factor-alpha (TNF-α) was analyzed, and histopathological evaluations were performed on days 7 and 14 post-wounding. Immunohistochemical analysis was conducted to quantify the expression of AMPK, Beclin-1, and p62. The results demonstrated that RB treatment significantly enhanced wound healing. Re-epithelialization, granulation tissue formation, and collagen deposition were markedly improved. Inflammation was substantially reduced, as evidenced by decreased TNF-α expression. The oxidative stress status was also ameliorated, with increased SOD activity and decreased levels of MDA and NO. Furthermore, RB treatment promoted autophagy activity, indicated by upregulated expression of AMPK and Beclin-1 and downregulated expression of p62.
Research Insights
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