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Evidence-Based Supplement Research
Evidence-Based Supplement Research

Intravenous Tirofiban After Tenecteplase in Acute Ischemic Stroke: The INSTANT Randomized Clinical Trial.

  • 2026-06-09
  • JAMA 335(22)
    • Xianghong Liu
    • Fan Zhang
    • Cong Zhang
    • Zongliang Li
    • Guangxiong Yuan
    • Deyan Kong
    • Shuhua Xie
    • Min Zhou
    • Jinchun Ye
    • Zhaohui Lai
    • Dan Li
    • Hailong Xu
    • Lei Chen
    • Fangqun Cheng
    • Yan Shi
    • Bin Chen
    • Weihua Hu
    • Huashi Liu
    • Yi Yin
    • Xinbo Deng
    • Zhiyong Xie
    • Chunying Li
    • Junfeng Shi
    • Donghuan Mei
    • Bin Liu
    • Bo Wang
    • Ruize Zhou
    • Jingjing Liu
    • Limin Shen
    • Hairong Hu
    • Genxiang Xiao
    • Xiaoyu Guan
    • Shanggui Yuan
    • Xunwei Lv
    • Shuiping Liang
    • Xiang Zeng
    • Yi Chen
    • Wenfeng Cao
    • Chong Zheng
    • Yuanlu Liu
    • Junda Li
    • Baozhu Guan
    • Tianmin Lai
    • Wei Sun
    • Hongliang Zeng
    • Jian Zhang
    • Shizhan Li
    • Hongfei Sang
    • Yamei Tang
    • Duolao Wang
    • Mengjie Lu
    • Johannes Kaesmacher
    • Vignan Yogendrakumar
    • Suyue Pan
    • Raul G Nogueira
    • Daojun Hong
    • Mayank Goyal
    • Götz Thomalla
    • Thanh N Nguyen
    • Jeffrey L Saver
    • Qingwu Yang
    • Zhongming Qiu
    • Guoyong Zeng

Study Design

Type
Randomized Controlled Trial (RCT)
Sample size
n = 359
Population
359 patients with acute ischemic stroke, without large or medium vessel occlusion or cardioembolic etiology, and with an insufficient clinical response to intravenous tenecteplase
Methods
Investigator-initiated, randomized, double-blind, placebo-controlled trial at 37 hospitals in China; intravenous tirofiban (0.3-μg/kg/min bolus over 30 min, then 0.075 μg/kg/min for up to 47.5 h) or matching placebo, initiated within 4 to 24 h after intravenous tenecteplase; oral antiplatelet therapy started 24 h after thrombolysis in placebo group and 44 h after in tirofiban group
Blinding
Double-blind
Duration
90 days
  • Large Human Trial
  • Rigorous Journal

Importance

Although recent trials have shown benefit with early tirofiban after thrombolysis, its efficacy remains uncertain in patients with acute ischemic stroke who do not have a large or medium vessel occlusion or a cardioembolic source and who show an inadequate clinical response to intravenous tenecteplase.

Objective

To assess the efficacy and safety of intravenous tirofiban administered after an inadequate response to intravenous tenecteplase in this specific patient population.

Design, setting, and participants

Investigator-initiated, randomized, double-blind, placebo-controlled trial conducted at 37 hospitals in China, enrolling 359 patients with acute ischemic stroke, without large or medium vessel occlusion or cardioembolic etiology, and with an insufficient clinical response to intravenous tenecteplase (defined as no significant change from baseline, neurological deterioration, or neurological fluctuation based on serial assessment of the National Institutes of Health Stroke Scale score within 4-24 hours after infusion). Recruitment took place between April 24, 2024, and July 16, 2025, with final follow-up on October 11, 2025.

Interventions

Intravenous tirofiban (n = 177), administered as a 0.3-μg/kg/min bolus over 30 minutes, followed by a continuous infusion of 0.075 μg/kg/min for up to 47.5 hours, or matching placebo (n = 182), initiated within 4 to 24 hours after intravenous tenecteplase. Oral antiplatelet therapy (aspirin and/or clopidogrel) was started 24 hours after thrombolysis in the placebo group and 44 hours after thrombolysis in the tirofiban group and continued in all patients through 90-day follow-up.

Main outcomes and measures

The primary outcome was an excellent outcome (defined as a score of 0 or 1 on the modified Rankin Scale; range, 0-6, with higher scores indicating more severe disability) at 90 days. Safety outcomes included incidence of symptomatic intracranial hemorrhage within 48 hours and 90-day mortality.

Results

Among 359 patients randomized (mean age, 66 years; 141 females [39.3%]), 358 (99.7%) completed the trial. An excellent outcome at 90 days was observed in 113 patients (63.8%) in the tirofiban group and in 95 patients (52.2%) in the placebo group (risk ratio, 1.22; 95% CI, 1.02-1.46; P = .03). Symptomatic intracranial hemorrhage within 48 hours occurred in 1 patient (0.9%) in the tirofiban group and no patients in the placebo group; 90-day mortality was 0.6% and 1.6%, respectively.

Conclusions and relevance

Among patients with acute ischemic stroke without large or medium vessel occlusion or a cardioembolic source who had an inadequate clinical response to intravenous tenecteplase, adjunctive intravenous tirofiban increased the likelihood of an excellent outcome at 90 days.

Trial registration

ClinicalTrials.gov Identifier: NCT05604638.

Research Insights

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