Isolation and Characterisation of Bacteriocin and Aggregation-Promoting Factor Production in Lactococcus lactis ssp. lactis BGBM50 Strain.
- 2020-09-30
- Food Technology and Biotechnology 53
- N. Mirković
- Z. Radulovic
- G. Uzelac
- J. Lozo
- D. Obradović
- L. Topisirović
- M. Kojić
- PubMed: 27904354
- DOI: 10.17113/FTB.53.02.15.3846
Study Design
- Methods
- In vitro experiment
Abstract
Lactococcus lactis ssp. lactis BGBM50, a producer of lactococcin G and aggregation-promoting factor, was isolated from selected lactic acid bacteria taken from semi-hard cheese traditionally produced in the village Žanjic, Montenegro. Strain BGBM50 harbours a number of plasmids of different sizes. Plasmid curing experiments showed that genes for bacteriocin production are located on pBM140, a plasmid 140 kb in length. PCR analysis with primers specific for lactococcin Q and G genes gave fragment of the expected size. In addition, after plasmid curing of strain BGBM50, different derivatives with altered phenotypes were obtained, among them BGBM50-34 strain, which retained bacteriocin synthesis but had enhanced aggregation ability.
Keywords: aggregation-promoting factor; bacteriocin lactococcin G; plasmid curing.