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Isolation, characterization and evaluation of probiotic lactic acid bacteria for potential use in animal production.

  • 2016-10
  • Research in Veterinary Science 108
    • Yaneisy García-Hernández
    • Tania Pérez-Sánchez
    • R. Boucourt
    • J. Balcázar
    • J. Nicoli
    • João Moreira-Silva
    • Z. Rodríguez
    • Héctor Fuertes
    • Odalys Núñez
    • N. Albelo
    • N. Halaihel


In livestock production, lactic acid bacteria (LAB) are the most common microorganisms used as probiotics. For such use, these bacteria must be correctly identified and characterized to ensure their safety and efficiency. In the present study, LAB were isolated from broiler excreta, where a fermentation process was used. Nine among sixteen isolates were identified by biochemical and molecular (sequencing of the 16S rRNA gene) methods as Lactobacillus crispatus (n=1), Lactobacillus pentosus (n=1), Weissella cibaria (n=1), Pediococcus pentosaceus (n=2) and Enterococcus hirae (n=4). Subsequently, these bacteria were characterized for their growth capabilities, lactic acid production, acidic pH and bile salts tolerance, cell surface hydrophobicity, antimicrobial susceptibility and antagonistic activity. Lactobacillus pentosus strain LB-31, which showed the best characteristics, was selected for further analysis. This strain was administered to broilers and showed the ability of modulating the immune response and producing beneficial effects on morpho-physiological, productive and health indicators of the animals.

Keywords: Broilers; Lactic acid bacteria; Lactobacillus pentosus; Probiotics.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactic Acid Bacteria YK-1Improved Immune ResponseBeneficial
Lactic Acid Bacteria YK-1Improved Production EfficiencyBeneficial

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