Isolation of a potential probiotic strain Bacillus amyloliquefaciensLPB-18 and identification of antimicrobial compounds responsible for inhibition of food-borne pathogens.
- 2022-12-12
- Food science & nutrition 11(5)
- Hedong Lu
- Panping Yang
- Mengyuan Zhong
- Muhammad Bilal
- Hai Xu
- Qihan Zhang
- Jiangnan Xu
- Naiguo Liang
- Shuai Liu
- Li Zhao
- Yuping Zhao
- Chengxin Geng
- PubMed: 37181301
- DOI: 10.1002/fsn3.3094
Study Design
- Type
- Review
- Methods
- Isolation of Bacillus strain from breeding soil of earthworms, identification by morphological and molecular analysis, antimicrobial testing via agar diffusion assay, RT-HPLC and MALDI-TOF MS analyses, antibiotic safety testing, and simulated gastrointestinal environment assays.
This study was carried out to screen a potential probiotic microbe with broad-spectrum antagonistic activity against food-borne pathogens and identify the antimicrobial compounds. Based on morphological and molecular analysis, a new Bacillus strain with the ability to produce effective antimicrobial agents was isolated from the breeding soil of earthworms and identified as having a close evolutionary footprint to Bacillus amyloliquefaciens. The antimicrobial substances produced by B. amyloliquefaciens show effective inhibition of Aspergillus flavus and Fusarium oxysporum in an agar diffusion assay. Antimicrobial agents were identified as a series of fengycin and its isoforms (fengycin A and fengycin B) after being submitted to RT-HPLC and MALDI-TOF MS analyses. To evaluate the probiotic activity of the B. amyloliquefaciens, antibiotic safety and viability of the isolated strain in a simulated gastrointestinal environment were carried out. The safety test result revealed that strain LPB-18 is susceptible to multiple common antibiotics. Moreover, acidic condition and bile salts assay were carried out, and the results revealed that it couble be a potential probiotic microbe B. amyloliquefaciens LPB-18 is good choice for biological strains in agricultural commodities and animal feedstuffs.