Laccase production by Monotospora sp., an endophytic fungus in Cynodon dactylon.
- 2006-03
- Bioresource Technology 97(5)
- Jian Wen Wang
- Junhua Wu
- W. Huang
- Renxiang Tan
- PubMed: 16006124
- DOI: 10.1016/J.BIORTECH.2005.03.025
Study Design
- Methods
- Controlled experimental study
Abstract
The effects of the carbon and nitrogen sources, initial pH and incubation temperature on laccase production by the endophytic fungus Monotospora sp. were evaluated. The optimal temperature and initial pH for laccase production by Monotospora sp. in submerged culture were found to be 30 degrees C and 8.5, respectively. Maltose (2 g l(-1)) and ammonium tartrate (10 g l(-1)) were the most suitable carbon and nitrogen source for laccase production. Under optimal culture medium, the maximum laccase activity was determined to be 13.55 U ml(-1), which was approximately four times higher than that in basal medium. This is the first report on laccase production by an endophytic fungus.