Lacticaseibacillus casei LH23 Suppressed HPV Gene Expression and Inhibited Cervical Cancer Cells
- 2021-10-02
- Probiotics and Antimicrobial Proteins 15(3)
- Shiyue Hu
- Y. Hao
- X. Zhang
- Yaping Yang
- Mei-ling Liu
- Nan Wang
- Tong-Cun Zhang
- Hongpeng He
- PubMed: 34599740
- DOI: 10.1007/s12602-021-09848-7
Abstract
Microbiota of lower female reproductive tract is special in its microorganism composition with Lactobacillus as the predominant bacteria. A few of Lactobacillus species have been identified to benefit the inhibition of inflammatory and malignant diseases. Lacticaseibacillus casei LH23 is a strain isolated from traditional fermented food and had been demonstrate to ameliorate DSS-induced colitis in mice. In the present study, effects of Lacticaseibacillus casei LH23 on cervical cancer cells were investigated. Supernatants of lysates and heat-inactivated Lacticaseibacillus casei LH23 were found to inhibit the expression of human papillomavirus genes E6/E7 which is the main causative factor of cervical cancer. With MTT, EdU staining, and TUNEL staining assays, Lacticaseibacillus casei LH23 was shown to suppress the proliferation and induced the apoptosis of cervical cancer cells. Additionally, with wound-healing and Western-blot assays, Lacticaseibacillus casei LH23 was shown to slowdown the migration of cervical cancer cells and altered the expression of metastasis-related genes. These results demonstrated the anti-cervical cancer potential of Lacticaseibacillus casei LH23.
Keywords: Apoptosis; Cervical cancer; HPV; Lactobacillus; Migration; Proliferation.
Research Insights
Supplement | Health Outcome | Effect Type | Effect Size |
---|---|---|---|
Lacticaseibacillus casei | Induced Cervical Cancer Cell Apoptosis | Beneficial | Large |
Lacticaseibacillus casei | Inhibited Cervical Cancer Cell Migration | Beneficial | Moderate |
Lacticaseibacillus casei | Reduced Cervical Cancer Cell Proliferation | Beneficial | Large |
Lacticaseibacillus casei | Reduced HPV Gene Expression | Beneficial | Large |