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Lacticaseibacillus paracasei sh2020 induced antitumor immunity and synergized with anti-programmed cell death 1 to reduce tumor burden in mice

  • 2022-03-08
  • Gut Microbes 14(1)
    • Shilong Zhang
    • Bing Han
    • Yu-qin Mao
    • Zheng-yan Zhang
    • Zhan-ming Li
    • Chao‐Yue Kong
    • You Wu
    • Guoqiang Chen
    • Li‐Shun Wang


The gut microbiota was emerging as critical regulatory elements in shaping the outcome of cancer immunotherapy. However, the underlying mechanisms by which the gut commensal species enhance antitumor immunity remain largely unexplored. Here, we show that the gut microbiota from healthy individuals conferred considerable sensitivity to anti-PD-1 in the colorectal cancer (CRC) tumor-bearing mice, whereas gut microbiota from CRC patients failed to do so. By 16S rRNA gene sequencing, we identified Lactobacillus that was significantly increased in the mice with good response to anti-PD-1, and significantly correlated with anti-tumor immunity. After a series of screening, we isolated a novel Lacticaseibacillus strain, named L. paracasei sh2020. L. paracasei sh2020 showed the most notable anti-tumor immunity in the mice with gut dysbiosis. Mechanistically, the antitumor immune response elicited by L. paracasei sh2020 was dependent on CD8+ T cell. In vitro and in vivo studies revealed that L. paracasei sh2020 stimulation triggered the upregulated expression of CXCL10 in the tumors and subsequently enhanced CD8+ T cell recruitment. Meanwhile, the modulation of gut microbiota caused by L. paracasei sh2020 enhanced its antitumor effect and gut barrier function. Overall, our study offered novel insights into the mechanism by which gut microbiota shaped the outcome of cancer immunotherapy and, more importantly, the novel strain L. paracasei sh2020 might serve as an easy and effective way to promote anti-PD-1 effect in clinical practice.

Keywords: Colorectal cancer; anti-PD-1; gut microbiota; immunotherapy; probiotics; tumor microenvironment.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lacticaseibacillus paracasei Lpc-37Enhanced Antitumor ImmunityBeneficial
Lacticaseibacillus paracasei Lpc-37Enhanced CD8+ T Cell RecruitmentBeneficial
Lacticaseibacillus paracasei Lpc-37Improved Intestinal Barrier FunctionBeneficial

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