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Lacticaseibacillus rhamnosus CA15 (DSM 33960) as a Candidate Probiotic Strain for Human Health

  • 2022-11-19
  • Nutrients 14(22)
    • A. Pino
    • Amanda Vaccalluzzo
    • C. Caggia
    • S. Balzaretti
    • L. Vanella
    • V. Sorrenti
    • Aki Ronkainen
    • R. Satokari
    • C. Randazzo


Lactobacilli with probiotic properties have emerged as promising tools for both the prevention and treatment of vaginal dysbiosis. The present study aimed to study the in vitro probiotic potential of the Lacticaseibacillus rhamnosus CA15 (DSM 33960) strain isolated from a healthy vaginal ecosystem. The strain was evaluated for both functional (antagonistic activity against pathogens; H2O2, organic acid, and lactic acid production; antioxidant and anti-inflammatory activities; ability to adhere to intestinal mucus and to both CaCo-2 and VK7/E6E7 cell lines; exopolysaccharide production; surface properties; and ability to survive during gastrointestinal transit) and safety (hemolytic, DNase, and gelatinase activities; mucin degradation ability; production of biogenic amines; and resistance to antimicrobials) characteristics. Data revealed that the tested strain was able to antagonize a broad spectrum of vaginal pathogens. In addition, the adhesion capacity to both vaginal and intestinal cell lines, as well as anti-inflammatory and antioxidant activities, was detected. The ability of the Lacticaseibacillus rhamnosus CA15 (DSM 33960) strain to survive under harsh environmental conditions occurring during the gastrointestinal passage suggests its possible oral delivery. Thus, in vitro data highlighted interesting probiotic properties of the CA15 (DSM 33960) strain, which could represent a valuable candidate for in vivo vaginal infections treatment.

Keywords: anti-inflammatory activity; antioxidant activity; functional features; lactobacilli; pathogen inhibition; safety.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lacticaseibacillus rhamnosusEnhanced Intestinal Epithelial Cell AdhesionBeneficial
Lacticaseibacillus rhamnosusIncreased Adhesion to Vaginal Cell LinesBeneficial
Lacticaseibacillus rhamnosusIncreased Antioxidant ActivityBeneficial
Lacticaseibacillus rhamnosusReduced Inflammation LevelsBeneficial
Lacticaseibacillus rhamnosusReduced Vaginal Pathogen LoadBeneficial
Lactobacillus rhamnosus DSM33560Improved Adhesion CapacityBeneficial
Lactobacillus rhamnosus DSM33560Increased Antioxidant ActivityBeneficial
Lactobacillus rhamnosus DSM33560Reduced Inflammation LevelsBeneficial
Lactobacillus rhamnosus DSM33560Reduced Pathogen GrowthBeneficial
Lactobacillus rhamnosus DSM33560Survival During Gastrointestinal TransitBeneficial

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