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Study Design

Population
C57BL/6 murine models
Methods
GUANKE (5×10^9 CFU/day) or exogenous linoleic acid (40 mg/kg) supplementation against influenza A virus (IAV)-induced pulmonary damage
Pulmonary inflammatory response represents a predominant complication arising from influenza virus infections. This investigation elucidates the protective efficacy of Lactiplantibacillus plantarum GUANKE (GUANKE) supplementation against influenza A virus (IAV)-induced pulmonary damage in C57BL/6 murine models, with particular emphasis on its mechanistic underpinnings. The results showed that the use of GUANKE (5 × 109 CFU/day) or exogenous linoleic acid (a metabolite of GUANKE) supplementation (40 mg/kg) significantly attenuated inflammatory cytokine secretion while counteracting virus-mediated downregulation of pulmonary barrier proteins. Mechanistic profiling revealed that GUANKE and GUANKE-derived linoleic acid modulates mitochondrial quality control through enhanced Parkin-dependent mitophagy coupled with restored mitochondrial oxidative phosphorylation (OXPHOS) capacity, thereby providing protection in IAV-infected mice.

Research Insights

SupplementDoseHealth OutcomeEffect TypeEffect SizeSource
Lactococcus lactis R1058Improved Lung Barrier FunctionBeneficial
Moderate
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counteracting virus-mediated downregulation of pulmonary barrier proteins

Lactococcus lactis R1058Reduced Lung InflammationBeneficial
Moderate
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GUANKE supplementation against influenza A virus (IAV)-induced pulmonary damage... significantly attenuated inflammatory cytokine secretion

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