Lactobacilli Strain Mixture Alleviates Bacterial Vaginosis through Antibacterial and Antagonistic Activity in Gardnerella vaginalis-Infected C57BL/6 Mice.

2022-02-20
Microorganisms 10(2)
- Soo-Im Choi
- GaYeong Won
- YongGyeong Kim
- Chang-Ho Kang
- Gun-Hee Kim

PubMed: 35208925
DOI: 10.3390/microorganisms10020471

Study Design

Population: HeLa cells and Gardnerella vaginalis (GV)-infected BV mice
Methods: A mixture of five lactobacilli strains (LM5) was tested in HeLa cells and GV-infected BV mice; LM5 was administered orally at 5 × 10^9 CFU/mouse

The present study investigated the anti-bacterial vaginitis (BV) effects of a mixture of five lactobacilli strains (LM5), containing equal amounts of Ligilactobacillus salivarius MG242, Limosilactobacillus fermentum MG901, Lactiplantibacillus plantarum MG989, Lacticaseibacillus paracasei MG4272, and Lacticaseibacillus rhamnosus MG4288), in HeLa cells and Gardnerella vaginalis (GV)-infected BV mice. All strains produced lactic acid and hydrogen peroxide, and were resistant to nonoxynol-9. LM5 significantly inhibited GV growth by 80%, exhibited good adhesion to HeLa cells, and significantly inhibited GV adhesion to these cells. In GV-infected mice, LM5 administered orally at 5 × 10⁹ CFU/mouse significantly inhibited GV proliferation in the vaginal tract and significantly reduced myeloperoxidase activity, pro-inflammatory cytokine (TNF-α, IL-1β, and IL-6) levels, and nitric oxide levels in vaginal tissue lysates. Histopathological analysis of vaginal tissues revealed that LM5 markedly suppressed the exfoliation of vaginal epithelial cells. Overall, these results suggest that LM5 might alleviate BV by direct antibacterial and antagonistic activity in vaginal tissues of GV-infected mice.

Research Insights

Lactobacillus rhamnosus→Improved Epithelial Integrity
Histopathological analysis of vaginal tissues revealed that LM5 markedly suppressed the exfoliation of vaginal epithelial cells.
Effect
Beneficial
Effect size
Moderate
Lactobacillus rhamnosus→Reduced Vaginal Inflammation
LM5 significantly reduced myeloperoxidase activity, pro-inflammatory cytokine (TNF-α, IL-1β, and IL-6) levels, and nitric oxide levels in vaginal tissue lysates.
Effect
Beneficial
Effect size
Moderate
Lactobacillus rhamnosus→Reduced Vaginal Pathogen Load
LM5 significantly inhibited GV proliferation in the vaginal tract and significantly reduced myeloperoxidase activity, pro-inflammatory cytokine (TNF-α, IL-1β, and IL-6) levels, and nitric oxide levels in vaginal tissue lysates.
Effect
Beneficial
Effect size
Large