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Abstract

This study aimed to investigate the combined effects of two most potent probiotic bacteria Lactobacillus acidophilus and Lactobacillus plantarum on overall health and immune status of freshwater crayfish, marron under laboratory conditions. A total of 36 marron were distributed into six different tanks and two different feeding groups, control and probiotic-fed group. After acclimation, control group was fed with basal diet while probiotic group was fed 109 CFU/mL per kg of bacterial supplemented feed for 60 days. The results showed no significant differences in weight gain, however, probiotic feed significantly enhanced some hemolymph parameters and biochemical composition of tail muscle. Histology data revealed better hepatopancreas health and higher microvilli counts in the marron gut fed probiotic diet. The probiotic bacteria triggered significant shift of microbial communities at different taxa level, mostly those reported as beneficial for crayfish. The probiotic diet also enriched the metabolic functions and genes associated with innate immune response of crayfish. Further correlation analysis revealed significant association of some taxa with increased activity for hemolymph and immune genes. Therefore, dietary Lactobacillus supplementation can modulate the overall health and immunity as well as gut microbial composition and interaction network between gut microbiota and immune system in crayfish.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus acidophilusAltered Gut Microbial CommunityBeneficial
Moderate
Lactobacillus acidophilusImproved Hemolymph ParametersBeneficial
Moderate
Lactobacillus acidophilusImproved Hepatopancreas HealthBeneficial
Moderate
Lactobacillus acidophilus HA-122Change in Vaginal Microbiota CompositionBeneficial
Moderate
Lactobacillus acidophilus HA-122Improved Hemolymph ParametersBeneficial
Moderate
Lactobacillus acidophilus HA-122Improved Hepatopancreas HealthBeneficial
Moderate
Lactobacillus acidophilus LA-02Altered Gut Microbiota CompositionBeneficial
Moderate
Lactobacillus acidophilus LA-02Improved Hemolymph ParametersBeneficial
Moderate
Lactobacillus acidophilus LA-02Improved Hepatopancreas HealthBeneficial
Moderate
Lactobacillus acidophilus La-14Improved Gut Microbiota CompositionBeneficial
Large
Lactobacillus acidophilus La-14Improved Hemolymph ParametersBeneficial
Moderate
Lactobacillus acidophilus La-14Improved Hepatopancreas HealthBeneficial
Moderate
Lactobacillus acidophilus MAK32L61AEnhanced Innate Immune FunctionBeneficial
Large
Lactobacillus acidophilus MAK32L61AImproved Hemolymph ParametersBeneficial
Moderate
Lactobacillus acidophilus MAK32L61AImproved Hepatopancreas HealthBeneficial
Moderate
Lactobacillus acidophilus NCIMB 30333Change in Vaginal Microbiota CompositionBeneficial
Moderate
Lactobacillus acidophilus NCIMB 30333Improved Hemolymph ParametersBeneficial
Moderate
Lactobacillus acidophilus NCIMB 30333Improved Muscle Biochemical CompositionBeneficial
Moderate
Lactobacillus acidophilus NCIMB 30333Increased Gut Microvilli CountsBeneficial
Moderate
Lactobacillus acidophilus R0418Improved Hemolymph ParametersBeneficial
Moderate
Lactobacillus acidophilus R0418Improved Microbial Community CompositionBeneficial
Moderate
Lactobacillus acidophilus R0418Improved Muscle Biochemical CompositionBeneficial
Moderate
Lactobacillus acidophilus R0418Increased Gut Microvilli CountBeneficial
Large
Lactobacillus acidophilus SD-5212Improved Hemolymph ParametersBeneficial
Moderate
Lactobacillus acidophilus SD-5212Improved Intestinal Microbial CompositionBeneficial
Large

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