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Lactobacillus acidophilus ATCC 4356 inhibits biofilm formation by C. albicans and attenuates the experimental candidiasis in Galleria mellonella

  • 2015-01-02
  • Virulence 6(1)
    • S. F. Vilela
    • J. Barbosa
    • R. D. Rossoni
    • J. D. Santos
    • M. C. A. Prata
    • A. Anbinder
    • A. O. Jorge
    • J. C. Junqueira

Abstract

Probiotic strains of Lactobacillus have been studied for their inhibitory effects on Candida albicans. However, few studies have investigated the effect of these strains on biofilm formation, filamentation and C. albicans infection. The objective of this study was to evaluate the influence of Lactobacillus acidophilus ATCC 4356 on C. albicans ATCC 18804 using in vitro and in vivo models. In vitro analysis evaluated the effects of L. acidophilus on the biofilm formation and on the capacity of C. albicans filamentation. For in vivo study, Galleria mellonella was used as an infection model to evaluate the effects of L. acidophilus on candidiasis by survival analysis, quantification of C. albicans CFU/mL, and histological analysis. The direct effects of L. acidophilus cells on C. albicans, as well as the indirect effects using only a Lactobacillus culture filtrate, were evaluated in both tests. The in vitro results showed that both L. acidophilus cells and filtrate were able to inhibit C. albicans biofilm formation and filamentation. In the in vivo study, injection of L. acidophilus into G. mellonella larvae infected with C. albicans increased the survival of these animals. Furthermore, the number of C. albicans CFU/mL recovered from the larval hemolymph was lower in the group inoculated with L. acidophilus compared to the control group. In conclusion, L. acidophilus ATCC 4356 inhibited in vitro biofilm formation by C. albicans and protected G. mellonella against experimental candidiasis in vivo.

Keywords: ATCC, American type culture collection; BHI, Brain heart infusion; CFU, colony-forming unit; Candida albicans; Galleria mellonella; HE, hematoxylin-eosin; Lactobacillus acidophilus; MRS, Man, Rogosa and Sharpe; NIH, National Institutes of Health; PAS, periodic acid-Schiff; PBS, phosphate buffered saline; SEM, Scanning electron microscopy; YNB, Yeast nitrogen base; biofilm; candidiasis; filamentation; pH, potential hydrogen ion; probiotic.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus acidophilusIncreased Survival Rate During CandidiasisBeneficial
Large
Lactobacillus acidophilusReduced Biofilm Formation by Candida albicansBeneficial
Moderate
Lactobacillus acidophilusReduced Candida albicans Colony-Forming UnitsBeneficial
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Lactobacillus acidophilus L-92Increased Survival Rate in Galleria mellonella Infected with Candida albicansBeneficial
Large
Lactobacillus acidophilus L-92Reduced Biofilm Formation by Candida albicansBeneficial
Moderate
Lactobacillus acidophilus La-14Reduced Biofilm Formation by Candida albicansBeneficial
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Lactobacillus acidophilus La-14Reduced Candida albicans Colony-Forming UnitsBeneficial
Moderate
Lactobacillus acidophilus MAK32L61AIncreased Survival Rate During CandidiasisBeneficial
Large
Lactobacillus acidophilus MAK32L61AReduced Biofilm Formation by Candida albicansBeneficial
Moderate
Lactobacillus acidophilus MAK32L61AReduced Candida albicans Colony-Forming UnitsBeneficial
Moderate
Lactobacillus acidophilus R0418Increased Survival Rate in Galleria mellonella Infected with Candida albicansBeneficial
Large
Lactobacillus acidophilus R0418Reduced Biofilm Formation by Candida albicansBeneficial
Moderate
Lactobacillus acidophilus R0418Reduced C. albicans Load in G. mellonellaBeneficial
Moderate
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