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Lactobacillus acidophilus NCFM and Lactiplantibacillus plantarum Lp-115 inhibit Helicobacter pylori colonization and gastric inflammation in a murine model

  • 2023-08-09
  • Frontiers in Cellular and Infection Microbiology 13
    • Siqi Shen
    • Feifei Ren
    • Haiming Qin
    • Ihtisham Bukhari
    • Jing Yang
    • Dafang Gao
    • A. Ouwehand
    • M. Lehtinen
    • Pengyuan Zheng
    • Yang Mi

Abstract

Purpose: To determine the role of Lactobacillus strains and their combinations in inhibiting the colonization of H. pylori and gastric mucosa inflammation.

Methods: Human gastric adenocarcinoma AGS cells were incubated with H. pylori and six probiotic strains (Lactobacillus acidophilus NCFM, L. acidophilus La-14, Lactiplantibacillus plantarum Lp-115, Lacticaseibacillus paracasei Lpc-37, Lacticaseibacillus rhamnosus Lr-32, and L. rhamnosus GG) and the adhesion ability of H. pylori in different combinations was evaluated by fluorescence microscopy and urease activity assay. Male C57BL/6 mice were randomly divided into five groups (uninfected, H. pylori, H. pylori+NCFM, H. pylori+Lp-115, and H. pylori+NCFM+Lp-115) and treated with two lactobacilli strains (NCFM and Lp-115) for six weeks. H. pylori colonization and tissue inflammation statuses were determined by rapid urease test, Hematoxylin-Eosin (HE) staining, immunohistochemistry, and qRT-PCR and ELISA.

Results: L. acidophilus NCFM, L. acidophilus La-14, L. plantarum Lp-115, L. paracasei Lpc-37, L. rhamnosus Lr-32, and L. rhamnosus GG reduced H. pylori adhesion and inflammation caused by H. pylori infection in AGS cells and mice. Among all probiotics L. acidophilus NCFM and L. plantarum, Lp-115 showed significant effects on the H. pylori eradication and reduction of inflammation in-vitro and in-vivo. Compared with the H. pylori infection group, the mRNA and protein expression levels of IL-8 and TNF-α in the six Lactobacillus intervention groups were significantly reduced. The changes in the urease activity (ureA and ureB) for 1-7h in each group showed that L. acidophilus NCFM, L. acidophilus La-14, L. plantarum Lp-115, and L. rhamnosus GG effectively reduced the colonization of H. pylori. We observed a higher ratio of lymphocyte and plasma cell infiltration into the lamina propria of the gastric mucosa and neutrophil infiltration in H. pylori+NCFM+Lp-115 mice. The infiltration of inflammatory cells in lamina propria of the gastric mucosa was reduced in the H. pylori+NCFM+Lp-115 group. Additionally, the expression of IFN-γ was decreased significantly in the NCFM and Lp-115 treated C57BL/6 mice.

Conclusions: L. acidophilus NCFM and L. plantarum Lp-115 can reduce the adhesion of H. pylori and inhibit the gastric inflammatory response caused by H. pylori infection.

Keywords: Helicobacter pylori; Lactobacillus; adhesion; inflammation; probiotic.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactiplantibacillus plantarum Lp-115Reduced Gastric InflammationBeneficial
Large
Lactiplantibacillus plantarum Lp-115Reduced Immune Cell InfiltrationBeneficial
Moderate
Lactiplantibacillus plantarum Lp-115Reduced Inflammatory MarkersBeneficial
Large
Lactiplantibacillus plantarum Lp-115Reduced Pathogen AdhesionBeneficial
Large
Lactiplantibacillus plantarum Lp-115Reduced Urease ActivityBeneficial
Large
Lactobacillus acidophilus NCFMReduced Helicobacter pylori ColonizationBeneficial
Large
Lactobacillus acidophilus NCFMReduced Inflammation in Gastric CellsBeneficial
Large
Lactobacillus plantarum Lp-115Reduced Gastric Mucosal InflammationBeneficial
Large
Lactobacillus plantarum Lp-115Reduced IFN-γ ExpressionBeneficial
Moderate
Lactobacillus plantarum Lp-115Reduced Immune Cell InfiltrationBeneficial
Moderate
Lactobacillus plantarum Lp-115Reduced Pathogen AdhesionBeneficial
Large

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