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Evidence-Based Supplement Research
Evidence-Based Supplement Research

Lactobacillus Acidophilus/Bifidobacterium Infantis Probiotics Are Beneficial to Extremely Low Gestational Age Infants Fed Human Milk

  • 2020-03-22
  • Nutrients 12(3)
    • Ingmar Fortmann
    • J. Marissen
    • Bastian Siller
    • J. Spiegler
    • Alexander Humberg
    • Kathrin Hanke
    • Kirstin Faust
    • Julia Pagel
    • Leila Eyvazzadeh
    • Kim Brenner
    • C. Roll
    • S. Pirr
    • D. Viemann
    • D. Stavropoulou
    • P. Henneke
    • Birte Tröger
    • Thorsten Körner
    • A. Stein
    • Christoph Derouet
    • M. Zemlin
    • C. Wieg
    • J. Rupp
    • E. Herting
    • W. Göpel
    • C. Härtel

Study Design

Type
Observational
Sample size
n = 7,516
Population
Preterm infants <29 weeks of gestation
Methods
Observational study

Abstract

Objective: To evaluate the nutrition-related effects of prophylactic Lactobacillus acidophilus/Bifidobacterium infantis probiotics on the outcomes of preterm infants <29 weeks of gestation that receive human milk and/or formula nutrition. We hypothesize that human-milk-fed infants benefit from probiotics in terms of sepsis prevention and growth.

Methods: We performed an observational study of the German Neonatal Network (GNN) over a period of six years, between 1 January, 2013 and 31 December, 2018. Prophylactic probiotic use of L. acidophilus/B. infantis was evaluated in preterm infants <29 weeks of gestation (n = 7516) in subgroups stratified to feeding type: (I) Exclusively human milk (HM) of own mother and/or donors (HM group, n = 1568), (II) HM of own mother and/or donor and formula (Mix group, n = 5221), and (III) exclusive exposure to formula (F group, n = 727). The effect of probiotics on general outcomes and growth was tested in univariate models and adjusted in linear/logistic regression models.

Results: 5954 (76.5%) infants received L. acidophilus/B. infantis prophylactically for the prevention of necrotizing enterocolitis (NEC). Probiotic use was associated with improved growth measures in the HM group (e.g., weight gain velocity in g/day: effect size B = 0.224; 95% CI: 2.82-4.35; p < 0.001) but not in the F group (effect size B = -0.06; 95% CI: -3.05-0.28; p = 0.103). The HM group had the lowest incidence of clinical sepsis (34.0%) as compared to the Mix group (35.5%) and the F group (40.0%). Only in the Mix group, probiotic supplementation proved to be protective against clinical sepsis (OR 0.69; 95% CI: 0.59-0.79; p < 0.001).

Conclusion: Our observational data indicate that the exposure to L. acidophilus/B. infantis probiotics may promote growth in exclusively HM-fed infants as compared to formula-fed infants. To exert a sepsis-preventive effect, probiotics seem to require human milk.

Keywords: growth failure; human milk; prematurity; probiotic prophylaxis; sepsis.

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