Lactobacillus brevis Alleviates DSS-Induced Colitis by Reprograming Intestinal Microbiota and Influencing Serum Metabolome in Murine Model
- 2019-09-18
- Frontiers in Physiology 10
- Sujuan Ding
- Yong Ma
- Gang Liu
- Wenxin Yan
- Hongmei Jiang
- Jun Fang
- PubMed: 31620010
- DOI: 10.3389/fphys.2019.01152
Abstract
The aim of this study was to examine the effects of Lactobacillus brevis on the microbial community and serum metabolome in colitis induced by dextran sulfate sodium (DSS). ICR mice were randomly distributed into three treatment groups: (i) L. brevis treatment alone (control), (ii) DSS administration alone, and (iii) treatment with L. brevis and DSS. Our results demonstrate that L. brevis treatment significantly alleviated DSS-induced body weight loss and colon inflammation. In addition, LC-MS analysis of serum metabolites revealed that L. brevis treatment increased the serum level of metabolites against inflammatory responses or oxidative stressors caused by DSS in the murine model. By detecting colonic microbiota, L. brevis increased colonic microbial diversity after challenging with DSS, and increased the relative abundance of Alloprevotella at genus, but Bacteroidales was reduced (P < 0.05). These result indicated that L. brevis could lower the severity of colitis induced by DSS via improving reprogramming the serum metabolome and intestinal microbiota. These findings suggest that the probiotic L. brevis may prevent tissue damage from colitis.
Keywords: Lactobacillus brevis; colitis; intestinal microbiota; metabolome; murine.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus brevis | Improved Gut Microbiota Diversity | Beneficial | Moderate |
| Lactobacillus brevis HA-112 | Improved Colonic Microbial Diversity | Beneficial | Moderate |
| Lactobacillus brevis HA-112 | Increased Serum Metabolites to Combat Inflammation | Beneficial | Moderate |
| Lactobacillus brevis HA-112 | Reduced Colitis Symptoms | Beneficial | Large |
| Lactobacillus brevis HA-112 | Reduced Relative Abundance of Bacteroidales | Neutral | Small |
| Lactobacillus brevis LB01 | Improved Gut Microbiota Diversity | Beneficial | Moderate |
| Lactobacillus brevis LB01 | Increased Alloprevotella Abundance | Beneficial | Small |
| Lactobacillus brevis LB01 | Increased Serum Anti-inflammatory Metabolites | Beneficial | Moderate |
| Lactobacillus brevis LB01 | Reduced Relative Abundance of Bacteroidales | Neutral | Small |
| Lactobacillus brevis Lbr-35 | Enhanced Serum Metabolite Levels Against Inflammation | Beneficial | Moderate |
| Lactobacillus brevis Lbr-35 | Improved Gut Microbiota Diversity | Beneficial | Moderate |
| Lactobacillus brevis Lbr-35 | Increased Alloprevotella Abundance | Beneficial | Small |
| Lactobacillus brevis Lbr-35 | Reduced Body Weight Loss | Beneficial | Large |
| Lactobacillus brevis Lbr-35 | Reduced Colonic Inflammation | Beneficial | Large |
| Lactobacillus brevis Lbr-35 | Reduced Relative Abundance of Bacteroidales | Neutral | Small |
| Lactobacillus brevis MAK11L82B | Elevated Serum Metabolites in Response to Inflammation | Beneficial | Moderate |
| Lactobacillus brevis MAK11L82B | Improved Gut Microbiota Diversity | Beneficial | Moderate |
| Lactobacillus brevis MAK11L82B | Increased Alloprevotella Abundance | Beneficial | Small |
| Lactobacillus brevis MAK11L82B | Reduced Colitis Symptoms | Beneficial | Large |
| Lactobacillus brevis SBC8803 | Improved Gut Microbiota Diversity | Beneficial | Moderate |
| Lactobacillus brevis SBC8803 | Increased Alloprevotella Abundance | Beneficial | Small |
| Lactobacillus brevis SBC8803 | Reduced Body Weight | Beneficial | Large |
| Lactobacillus brevis SBC8803 | Reduced Colitis Severity | Beneficial | Large |
| Lactobacillus brevis SBC8803 | Reduced Colonic Inflammation | Beneficial | Large |
| Lactobacillus brevis SBC8803 | Reduced Relative Abundance of Bacteroidales | Neutral | Small |
| Lactobacillus brevis SBC8803 | Reduced Tissue Damage from Colitis | Beneficial | Large |
| Lactobacillus brevis SD-5214 | Improved Gut Microbiota Diversity | Beneficial | Moderate |
| Lactobacillus brevis SD-5214 | Reduced Colitis Severity | Beneficial | Large |
| Lactobacillus brevis SD-5214 | Reduced Severity of DSS-Induced Colitis | Beneficial | Large |
| Lactobacillus brevis SD5214 | Increased Serum Anti-inflammatory Metabolites | Beneficial | Moderate |
| Lactobacillus brevis UALbr-02 | Improved Gut Microbiota Diversity | Beneficial | Moderate |
| Lactobacillus brevis UALbr-02 | Increased Alloprevotella Abundance | Beneficial | Small |
| Lactobacillus brevis UALbr-02 | Increased Anti-Inflammatory Marker Levels | Beneficial | Moderate |
| Lactobacillus brevis UALbr-02 | Reduced Body Weight Loss | Beneficial | Large |
| Lactobacillus brevis UALbr-02 | Reduced Colonic Inflammation | Beneficial | Large |
| Lactobacillus brevis UALbr-02 | Reduced Relative Abundance of Bacteroidales | Neutral | Small |
| Lactobacillus brevis VPro 18 | Reduced DSS-Induced Body Weight Loss | Beneficial | Moderate |