Lactobacillus brevis Strains from Fermented Aloe vera Survive Gastroduodenal Environment and Suppress Common Food Borne Enteropathogens
- 2014-03-05
- PLoS ONE 9(3)
- Young‐Wook Kim
- Young-ju Jeong
- Ah-Young Kim
- H. Son
- Jong-Am Lee
- Cheong-Hwan Jung
- Chae-Hyun Kim
- Jaeman Kim
- PubMed: 24598940
- DOI: 10.1371/journal.pone.0090866
Abstract
Five novel Lactobacillus brevis strains were isolated from naturally fermented Aloe vera leaf flesh. Each strain was identified by Random Amplified Polymorphic DNA (RAPD) analysis and 16S rRNA sequence comparison. These strains were highly tolerant to acid, surviving in pH2.5 for up to 4 hours, and resistant to 5% bile salts at 37°C for 18 hours. Due to its tolerance to acid and bile salts, one strain passed through the gastric barrier and colonised the intestine after oral administration. All five strains inhibited the growth of many harmful enteropathogens without restraining most of normal commensals in the gut and hence named POAL (Probiotics Originating from Aloe Leaf) strains. Additionally, each strain exhibited discriminative resistance to a wide range of antibiotics. The L. brevis POAL strains, moreover, expressed high levels of the glutamate decarboxylase (GAD) gene which produces a beneficial neurotransmitter, γ-aminobutyric acid (GABA). These characteristics in all suggest that the novel L. brevis strains should be considered as potential food additives and resources for pharmaceutical research.
Research Insights
Supplement | Health Outcome | Effect Type | Effect Size |
---|---|---|---|
Lactobacillus brevis | Colonization of the Intestine | Beneficial | Moderate |
Lactobacillus brevis | Increased GABA Production | Beneficial | Large |
Lactobacillus brevis | Reduced Pathogenic Bacteria Levels | Beneficial | Large |
Lactobacillus brevis HA-112 | Colonization of the Intestine | Beneficial | Moderate |
Lactobacillus brevis HA-112 | Improved Survival in Gastroduodenal Environment | Beneficial | Large |
Lactobacillus brevis HA-112 | Increased γ-Aminobutyric Acid (GABA) Production | Beneficial | Moderate |
Lactobacillus brevis HA-112 | Reduced Pathogenic Bacteria Levels | Beneficial | Large |
Lactobacillus brevis LB01 | Efficient Intestinal Colonization | Beneficial | Large |
Lactobacillus brevis LB01 | Improved Survival in Gastroduodenal Environment | Beneficial | Large |
Lactobacillus brevis LB01 | Increased γ-Aminobutyric Acid (GABA) Production | Beneficial | Moderate |
Lactobacillus brevis LB01 | Reduced Enteropathogen Activity | Beneficial | Large |
Lactobacillus brevis Lbr-35 | Improved Survival in Gastroduodenal Environment | Beneficial | Large |
Lactobacillus brevis Lbr-35 | Increased γ-Aminobutyric Acid (GABA) Production | Beneficial | Moderate |
Lactobacillus brevis Lbr-35 | Reduced Enteropathogen Growth | Beneficial | Moderate |
Lactobacillus brevis MAK11L82B | Colonization of the Intestine | Beneficial | Moderate |
Lactobacillus brevis MAK11L82B | Improved Survival in Gastroduodenal Environment | Beneficial | Large |
Lactobacillus brevis MAK11L82B | Increased γ-Aminobutyric Acid (GABA) Production | Beneficial | Moderate |
Lactobacillus brevis MAK11L82B | Reduced Pathogenic Bacteria Levels | Beneficial | Large |
Lactobacillus brevis SBC8803 | Improved Survival in Gastroduodenal Environment | Beneficial | Large |
Lactobacillus brevis SBC8803 | Increased γ-Aminobutyric Acid (GABA) Production | Beneficial | Moderate |
Lactobacillus brevis SBC8803 | Reduced Enteropathogenic Burden | Beneficial | Large |
Lactobacillus brevis SD-5214 | Improved Survival in Gastroduodenal Environment | Beneficial | Large |
Lactobacillus brevis SD-5214 | Increased Intestinal Colonization | Beneficial | Large |
Lactobacillus brevis SD-5214 | Increased γ-Aminobutyric Acid (GABA) Production | Beneficial | Moderate |
Lactobacillus brevis SD-5214 | Reduced Pathogenic Bacteria Levels | Beneficial | Large |
Lactobacillus brevis UALbr-02 | Altered Glutamate Decarboxylase Gene Expression | Beneficial | Moderate |
Lactobacillus brevis UALbr-02 | Improved Survival in Gastroduodenal Environment | Beneficial | Large |
Lactobacillus brevis UALbr-02 | Increased Intestinal Colonization | Beneficial | Large |
Lactobacillus brevis UALbr-02 | Reduced Enteropathogen Activity | Beneficial | Large |
Lactobacillus brevis VPro 18 | Increased Production of Beneficial Neurotransmitter GABA | Beneficial | Moderate |