Lactobacillus paracasei CCFM1223 Protects against Lipopolysaccharide-Induced Acute Liver Injury in Mice by Regulating the “Gut–Liver” Axis
- 2022-06-30
- Microorganisms 10(7)
- Weiling Guo
- Bingyong Mao
- Xin Tang
- Qiuxiang Zhang
- Jianxin Zhao
- Shumao Cui
- Hao Zhang
- PubMed: 35889040
- DOI: 10.3390/microorganisms10071321
Abstract
Background: Lactobacillus paracasei CCFM1223, a probiotic previously isolated from the healthy people's intestine, exerts the beneficial influence of preventing the development of inflammation.
Methods: The aim of this research was to explore the beneficial effects of L. paracasei CCFM1223 to prevent lipopolysaccharide (LPS)-induced acute liver injury (ALI) and elaborate on its hepatoprotective mechanisms.
Results: L. paracasei CCFM1223 pretreatment remarkably decreased the activities of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in mice with LPS treatment and remarkably recovered LPS-induced the changes in inflammatory cytokines (tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), interleukin (IL)-1β, IL-6, IL-17, IL-10, and LPS) and antioxidative enzymes activities (total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT)). Metagenomic analysis showed that L. paracasei CCFM1223 pretreatment remarkably increased the relative abundance of Catabacter compared with the LPS group but remarkably reduced the relative abundance of [Eubacterium] xylanophilum__group, ASF356, Lachnospiraceae__NK4A136__group, and Lachnoclostridium, which is closely associated with the inflammation cytokines and antioxidative enzymes. Furthermore, L. paracasei CCFM1223 pretreatment remarkably increased the colonic, serum, and hepatic IL-22 levels in ALI mice. In addition, L. paracasei CCFM1223 pretreatment remarkably down-regulated the hepatic Tlr4 and Nf-kβ transcriptions and significantly up-regulated the hepatic Tlr9, Tak1, Iκ-Bα, and Nrf2 transcriptions in ALI mice.
Conclusions: L. paracasei CCFM1223 has a hepatoprotective function in ameliorating LPS-induced ALI by regulating the "gut-liver" axis.
Keywords: Lactobacillus paracasei CCFM1223; acute liver injury; gut–liver axis; intestinal microbiota; mRNA expression.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus paracasei LPC09 | Elevated IL-22 Levels | Beneficial | Large |
| Lactobacillus paracasei LPC09 | Improved Antioxidative Enzyme Activities | Beneficial | Large |
| Lactobacillus paracasei LPC09 | Improved Beneficial Intestinal Microbiota | Beneficial | Moderate |
| Lactobacillus paracasei LPC09 | Modulated Hepatic mRNA Expression | Beneficial | Moderate |
| Lactobacillus paracasei LPC09 | Reduced Harmful Gut Microbiota | Beneficial | Moderate |
| Lactobacillus paracasei LPC09 | Reduced Inflammatory Cytokine Levels | Beneficial | Large |
| Lactobacillus paracasei LPC09 | Reduced Liver Enzyme Levels | Beneficial | Large |