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Lactobacillus paracasei K5 displays adhesion, anti-proliferative activity and apoptotic effects in human colon cancer cells.

  • 2018-12-07
  • Beneficial Microbes 9(6)
    • P. Chondrou
    • A. Karapetsas
    • D. Kiousi
    • D. Tsela
    • A. Tiptiri-Kourpeti
    • I. Anestopoulos
    • I. Kotsianidis
    • E. Bezirtzoglou
    • Aglaia Pappa
    • A. Galanis

Abstract

Lactobacillus paracasei K5 is a lactic acid bacteria (LAB) strain, isolated recently from feta-type cheese. Its probiotic potential has been demonstrated in a series of established in vitro tests. Moreover, incorporation of L. paracasei K5 as starter culture offered organoleptic and technological advantages to novel fermented food products. In the present study, further investigation of the potential probiotic activity of L. paracasei K5 was performed and its mechanisms of action were investigated. Employing quantitative analysis and confocal, fluorescent microscopy the adhesion properties of the above strain were studied. L. paracasei K5 displayed efficient adherence capacity to Caco-2 colon cancer cells, similarly to the reference strains Lactobacillus casei ATCC 393 and Lactobacillus rhamnosus GG. Moreover, treatment of Caco-2 cells with L. paracasei K5 inhibited cell proliferation in a time-and dose-dependent manner. The anti-proliferative effects appear to be mediated through induction of apoptosis via modulation of expression of specific Bcl-2 family proteins. These results elucidate the mechanisms of action of L. paracasei K5 and enhance its potential probiotic activity.

Keywords: adhesion; apoptosis; probiotics; proliferation.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus paracaseiImproved Colon Cell AdhesionBeneficial
Moderate
Lactobacillus paracaseiIncreased Apoptosis in Colon Cancer CellsBeneficial
Large
Lactobacillus paracaseiReduced Colon Cancer Cell ProliferationBeneficial
Large
Lactobacillus paracasei IMC 502Enhanced Adhesion to Colon CellsBeneficial
Moderate
Lactobacillus paracasei IMC 502Induced ApoptosisBeneficial
Moderate
Lactobacillus paracasei IMC 502Reduced Cell ProliferationBeneficial
Moderate
Lactobacillus paracasei LPC09Increased Apoptosis in Colorectal Cancer CellsBeneficial
Large
Lactobacillus paracasei LPC09Reduced Colon Cancer Cell ProliferationBeneficial
Large
Lactobacillus paracasei MCC1849Induced ApoptosisBeneficial
Moderate
Lactobacillus paracasei MCC1849Reduced Cell ProliferationBeneficial
Moderate
Lactobacillus paracasei subsp. paracasei UALpc-04Induced ApoptosisBeneficial
Moderate
Lactobacillus paracasei subsp. paracasei UALpc-04Reduced Cell ProliferationBeneficial
Moderate
Lactobacillus paracasei subsp. paracasei UALs-04Increased Cancer Cell ApoptosisBeneficial
Moderate
Lactobacillus paracasei subsp. paracasei UALs-04Reduced Cancer Cell ProliferationBeneficial
Moderate
Lactobacillus paracasei VPro 224Improved Colon Cell AdhesionBeneficial
Moderate
Lactobacillus paracasei VPro 224Induced ApoptosisBeneficial
Moderate
Lactobacillus paracasei VPro 224Reduced Cell ProliferationBeneficial
Moderate
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