Lactobacillus paracasei K5 displays adhesion, anti-proliferative activity and apoptotic effects in human colon cancer cells.
- 2018-12-07
- Beneficial Microbes 9(6)
- P. Chondrou
- A. Karapetsas
- D. Kiousi
- D. Tsela
- A. Tiptiri-Kourpeti
- I. Anestopoulos
- I. Kotsianidis
- E. Bezirtzoglou
- Aglaia Pappa
- A. Galanis
- PubMed: 30353740
- DOI: 10.3920/BM2017.0183
Abstract
Lactobacillus paracasei K5 is a lactic acid bacteria (LAB) strain, isolated recently from feta-type cheese. Its probiotic potential has been demonstrated in a series of established in vitro tests. Moreover, incorporation of L. paracasei K5 as starter culture offered organoleptic and technological advantages to novel fermented food products. In the present study, further investigation of the potential probiotic activity of L. paracasei K5 was performed and its mechanisms of action were investigated. Employing quantitative analysis and confocal, fluorescent microscopy the adhesion properties of the above strain were studied. L. paracasei K5 displayed efficient adherence capacity to Caco-2 colon cancer cells, similarly to the reference strains Lactobacillus casei ATCC 393 and Lactobacillus rhamnosus GG. Moreover, treatment of Caco-2 cells with L. paracasei K5 inhibited cell proliferation in a time-and dose-dependent manner. The anti-proliferative effects appear to be mediated through induction of apoptosis via modulation of expression of specific Bcl-2 family proteins. These results elucidate the mechanisms of action of L. paracasei K5 and enhance its potential probiotic activity.
Keywords: adhesion; apoptosis; probiotics; proliferation.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus paracasei | Improved Colon Cell Adhesion | Beneficial | Moderate |
| Lactobacillus paracasei | Increased Apoptosis in Colon Cancer Cells | Beneficial | Large |
| Lactobacillus paracasei | Reduced Colon Cancer Cell Proliferation | Beneficial | Large |
| Lactobacillus paracasei IMC 502 | Enhanced Adhesion to Colon Cells | Beneficial | Moderate |
| Lactobacillus paracasei IMC 502 | Induced Apoptosis | Beneficial | Moderate |
| Lactobacillus paracasei IMC 502 | Reduced Cell Proliferation | Beneficial | Moderate |
| Lactobacillus paracasei LPC09 | Increased Apoptosis in Colorectal Cancer Cells | Beneficial | Large |
| Lactobacillus paracasei LPC09 | Reduced Colon Cancer Cell Proliferation | Beneficial | Large |
| Lactobacillus paracasei MCC1849 | Induced Apoptosis | Beneficial | Moderate |
| Lactobacillus paracasei MCC1849 | Reduced Cell Proliferation | Beneficial | Moderate |
| Lactobacillus paracasei subsp. paracasei UALpc-04 | Induced Apoptosis | Beneficial | Moderate |
| Lactobacillus paracasei subsp. paracasei UALpc-04 | Reduced Cell Proliferation | Beneficial | Moderate |
| Lactobacillus paracasei subsp. paracasei UALs-04 | Increased Cancer Cell Apoptosis | Beneficial | Moderate |
| Lactobacillus paracasei subsp. paracasei UALs-04 | Reduced Cancer Cell Proliferation | Beneficial | Moderate |
| Lactobacillus paracasei VPro 224 | Improved Colon Cell Adhesion | Beneficial | Moderate |
| Lactobacillus paracasei VPro 224 | Induced Apoptosis | Beneficial | Moderate |
| Lactobacillus paracasei VPro 224 | Reduced Cell Proliferation | Beneficial | Moderate |