Skip to main content

Abstract

We investigated the Lactobacillus plantarum bacteriocin plantaricin EF (PlnEF) system for its contributions to L. plantarum mediated benefits in a mouse model of diet-induced obesity. C57BL/6J mice on a high-fat diet (HFD) were administered a rifampicin resistant mutant of L. plantarum NCMIB8826 (NICMB8826-R) or an isogenic Δ_plnEFI_ mutant strain, LM0419, every 48 h for nine weeks. Mice fed wild-type L. plantarum, but not LM0419, reduced their consumption of the HFD starting three weeks into the study and exhibited an overall 10% reduction in weight gain. The responses were independent of glucose homeostasis, as both NCMIB8826-R and LM0419 fed mice had improved oral glucose tolerance compared to sham controls. Although bacteriocins have antibacterial properties, the ileal, cecal, and fecal microbiota and cecocolic metabolomes were unchanged between mice fed either wild-type L. plantarum or the Δ_plnEFI_ mutant. Instead, only mice fed NCMIB8826-R showed an increased production of ZO-1 in ileal tissues. To verify a potential role for the plantaricin EF system in supporting intestinal epithelial function, synthesized PlnEF peptides were applied to Caco-2 cell monolayers challenged with TNF-α and IFN-γ. The combination of PlnE and PlnF were required to prevent sustained cytokine-induced losses to Caco-2 cell para- and transcellular permeability and elevated IL-8 levels. In conclusion, this study shows that probiotic L. plantarum ameliorates the effects of obesogenic diets through a mechanism that involves the plantaricin EF system and likely includes L. plantarum - induced fortification of the intestinal epithelium.

Keywords: Probiotics; bacteriocins; high-fat; intestinal barrier integrity; lactobacillus; metabolome; microbiome; obesity; weight gain.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus plantarum 14DImproved Glucose ToleranceBeneficial
Moderate
Lactobacillus plantarum 14DIncreased ZO-1 Production in Ileal TissuesBeneficial
Moderate
Lactobacillus plantarum 14DMaintained Colonic Epithelial Barrier IntegrityBeneficial
Moderate
Lactobacillus plantarum 14DReduced Weight GainBeneficial
Moderate
Lactobacillus plantarum DSM 6596Improved Glucose ToleranceBeneficial
Moderate
Lactobacillus plantarum DSM 6596Increased ZO-1 Production in Ileal TissuesBeneficial
Moderate
Lactobacillus plantarum DSM 6596Maintained Colonic Epithelial Barrier IntegrityBeneficial
Moderate
Lactobacillus plantarum DSM 6596Reduced Weight GainBeneficial
Moderate
⬆ Back to top
Pillser
Supplement Research and Comparison Website: evidence-based information about supplements, their benefits, potential risks, and their efficacy.
Receive updates about our products, services, sales, and special offers. Unsubscribe anytime. See our Privacy Policy for details on how we handle your information.

Join Our Community

Use support@pillser.com to get in touch.