Lactobacillus plantarum L15 Alleviates Colitis by Inhibiting LPS-Mediated NF-κB Activation and Ameliorates DSS-Induced Gut Microbiota Dysbiosis
- 2020-10-02
- Frontiers in Immunology 11
- P. Yu
- Chuxin Ke
- Jiaxin Guo
- Xiuling Zhang
- Bailiang Li
- PubMed: 33123156
- DOI: 10.3389/fimmu.2020.575173
Abstract
Previous studies have suggested that the Lactobacillus plantarum bacteria strain could be effective in ulcerative colitis (UC) management. However, its effects are strain-specific and the related mechanisms for its attenuating effects on UC remain unclear. This study aimed to elucidate the underlying mechanisms for the protective effect of L. plantarum on UC. Firstly, 15 L. plantarum strains were screened for potential probiotic characteristics with good tolerance to simulated human gastrointestinal transit and adhesion. Secondly, the inflammatory response of selected strains to the Caco-2 cells induced by lipopolysaccharide (LPS) was measured. Finally, an in vivo mouse model induced by dextran sulfate sodium (DSS) was used to assess the beneficial effects and likely action mechanisms the successfully screened in vitro strain, L. plantarum L15. In vitro results showed that L. plantarum L15 possessed the highest gastrointestinal transit tolerance, adhesion and reduction of pro-inflammatory abilities compared to the other screened strains. In vivo, high dose of L. plantarum L15 supplementation increased the body weight, colon length and anti-inflammatory cytokine production. Pro-inflammatory cytokine production, disease activity index (DAI) levels and myeloperoxidase (MPO) parameters decreased using this strain. In addition, L. plantarum L15 alleviated the histopathological changes in colon, modulated the gut microbiota, and decreased LPS secretion. The activities of this strain down-regulated the expression of TLR4 and MyD88 genes as well as genes associated with NF-κB signaling pathway. Our findings present L. plantarum L15 as a new probiotic, with promising application for UC management.
Keywords: DSS-colitis; Lactobacillus plantarum; NF-κB signaling; gut microbiota; lipopolysaccharide (LPS).
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactiplantibacillus plantarum LP-1 | Alleviated Histopathological Changes in the Colon | Beneficial | Moderate |
| Lactiplantibacillus plantarum LP-1 | Altered Gut Microbiota | Beneficial | Moderate |
| Lactiplantibacillus plantarum LP-1 | Increased Anti-Inflammatory Cytokine Levels | Beneficial | Moderate |
| Lactiplantibacillus plantarum LP-1 | Increased Body Weight | Beneficial | Moderate |
| Lactiplantibacillus plantarum LP-1 | Increased Colon Length | Beneficial | Moderate |
| Lactiplantibacillus plantarum LP-1 | Reduced Disease Activity Index (DAI) Levels | Beneficial | Moderate |
| Lactiplantibacillus plantarum LP-1 | Reduced LPS Secretion | Beneficial | Moderate |
| Lactiplantibacillus plantarum LP-1 | Reduced Myeloperoxidase Levels | Beneficial | Moderate |
| Lactiplantibacillus plantarum LP-1 | Reduced Pro-Inflammatory Cytokines | Beneficial | Moderate |
| Lactobacillus plantarum DSM 6596 | Alleviated Histopathological Changes in the Colon | Beneficial | Moderate |
| Lactobacillus plantarum DSM 6596 | Altered Gut Microbiota | Beneficial | Moderate |
| Lactobacillus plantarum DSM 6596 | Increased Anti-Inflammatory Cytokine Levels | Beneficial | Moderate |
| Lactobacillus plantarum DSM 6596 | Increased Body Weight | Beneficial | Moderate |
| Lactobacillus plantarum DSM 6596 | Increased Colon Length | Beneficial | Moderate |
| Lactobacillus plantarum DSM 6596 | Reduced Disease Activity Index (DAI) Levels | Beneficial | Moderate |
| Lactobacillus plantarum DSM 6596 | Reduced LPS Secretion | Beneficial | Moderate |
| Lactobacillus plantarum DSM 6596 | Reduced Myeloperoxidase Levels | Beneficial | Moderate |
| Lactobacillus plantarum DSM 6596 | Reduced Pro-Inflammatory Cytokines | Beneficial | Moderate |
| Lactobacillus plantarum KABP™-061 | Alleviated Histopathological Changes in the Colon | Beneficial | Moderate |
| Lactobacillus plantarum KABP™-061 | Altered Gut Microbiota | Beneficial | Moderate |
| Lactobacillus plantarum KABP™-061 | Improved Body Weight | Beneficial | Moderate |
| Lactobacillus plantarum KABP™-061 | Increased Anti-Inflammatory Cytokine Levels | Beneficial | Moderate |
| Lactobacillus plantarum KABP™-061 | Increased Colon Length | Beneficial | Moderate |
| Lactobacillus plantarum KABP™-061 | Reduced Disease Activity Index (DAI) Levels | Beneficial | Moderate |
| Lactobacillus plantarum KABP™-061 | Reduced LPS Secretion | Beneficial | Moderate |
| Lactobacillus plantarum KABP™-061 | Reduced Myeloperoxidase Levels | Beneficial | Moderate |
| Lactobacillus plantarum KABP™-061 | Reduced Pro-Inflammatory Cytokines | Beneficial | Moderate |
| Lactobacillus plantarum L-137 | Alleviated Histopathological Changes in the Colon | Beneficial | Moderate |
| Lactobacillus plantarum L-137 | Altered Gut Microbiota | Beneficial | Moderate |
| Lactobacillus plantarum L-137 | Increased Anti-Inflammatory Cytokine Levels | Beneficial | Moderate |
| Lactobacillus plantarum L-137 | Increased Body Weight | Beneficial | Moderate |
| Lactobacillus plantarum L-137 | Increased Colon Length | Beneficial | Moderate |
| Lactobacillus plantarum L-137 | Reduced Disease Activity Index (DAI) Levels | Beneficial | Moderate |
| Lactobacillus plantarum L-137 | Reduced LPS Secretion | Beneficial | Moderate |
| Lactobacillus plantarum L-137 | Reduced Myeloperoxidase Levels | Beneficial | Moderate |
| Lactobacillus plantarum L-137 | Reduced Pro-Inflammatory Cytokines | Beneficial | Moderate |
| Lactobacillus plantarum LP-01 | Alleviated Histopathological Changes in the Colon | Beneficial | Moderate |
| Lactobacillus plantarum LP-01 | Altered Gut Microbiota | Beneficial | Moderate |
| Lactobacillus plantarum LP-01 | Increased Anti-Inflammatory Cytokine Levels | Beneficial | Moderate |
| Lactobacillus plantarum LP-01 | Increased Body Weight | Beneficial | Moderate |
| Lactobacillus plantarum LP-01 | Increased Colon Length | Beneficial | Moderate |
| Lactobacillus plantarum LP-01 | Reduced Disease Activity Index (DAI) Levels | Beneficial | Moderate |
| Lactobacillus plantarum LP-01 | Reduced LPS Secretion | Beneficial | Moderate |
| Lactobacillus plantarum LP-01 | Reduced Myeloperoxidase Levels | Beneficial | Moderate |
| Lactobacillus plantarum LP-01 | Reduced Pro-Inflammatory Cytokines | Beneficial | Moderate |
| Lactobacillus plantarum Lp-115 | Alleviated Histopathological Changes in the Colon | Beneficial | Moderate |
| Lactobacillus plantarum Lp-115 | Down-Regulated Genes in NF-κB Signaling Pathway | Beneficial | Moderate |
| Lactobacillus plantarum Lp-115 | Increased Anti-Inflammatory Cytokine Levels | Beneficial | Moderate |
| Lactobacillus plantarum Lp-115 | Increased Body Weight | Beneficial | Moderate |
| Lactobacillus plantarum Lp-115 | Increased Colon Length | Beneficial | Moderate |
| Lactobacillus plantarum Lp-115 | Modulated Gut Microbiota | Beneficial | Large |
| Lactobacillus plantarum Lp-115 | Reduced Disease Activity Index (DAI) Levels | Beneficial | Moderate |
| Lactobacillus plantarum Lp-115 | Reduced LPS Secretion | Beneficial | Moderate |
| Lactobacillus plantarum Lp-115 | Reduced Myeloperoxidase Levels | Beneficial | Moderate |
| Lactobacillus plantarum Lp-115 | Reduced Pro-Inflammatory Cytokines | Beneficial | Moderate |