Lactobacillus reuteri DSM 17938 Improves Intestinal Motility in Mice With Postoperative Ileus by Modulating Gut Microbiota and Macrophage.
- 2026-02
- Neurogastroenterology and motility 38(2)
- Jingyi Tang
- Yingnan Hu
- Dian Yu
- Baoqing Liu
- Wenjun Xu
- Weihan Zhu
- Yanting Xia
- Zhipeng Zheng
- Wei Zhang
- PubMed: 41672915
- DOI: 10.1111/nmo.70260
Background
Postoperative ileus (POI) is a common complication after abdominal operation, which is characterized by delayed gastrointestinal motility that can lead to prolonged hospitalization. Previous studies have demonstrated that gut microbiota and macrophages are involved in POI pathogenesis, but the underlying mechanism and the role of probiotics in it are still unclear. This work investigated the prophylactic effect of Lactobacillus reuteri (L. reuteri) DSM 17938 administration on POI mice.Methods
Mice were supplemented with antibiotics or L. reuteri DSM 17938 for two weeks before intestinal manipulation surgery. One day after surgery, the small intestine transit rate was evaluated. Samples were collected to determine the intestinal macrophages, histology, cytokines, gut microbiota, and fecal metabolome.Key result
Results showed that gut microbiota alteration contributed to POI. L. reuteri DSM 17938 increased intestinal motility of POI mice, maintained the proportion of intestinal CX3CR1+ macrophage, reduced M1 macrophage, and modulated macrophage-associated cytokines. Additionally, L. reuteri DSM 17938 slightly alleviated gut metabolic disorders but had an effect on a portion of metabolites of fecal tryptophan metabolism, modulated gut microbiota composition and function, and specifically suppressed bacterial invasion of epithelial cells and the expansion of Escherichia coli, which had a negative correlation with the small intestine transit rate in POI mice.Conclusions and inferences
Our findings indicate that L. reuteri DSM 17938 is a promising probiotic for the prevention of POI, but it still needs further clinical validation.Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus reuteri DSM 17938 | Improved Intestinal Motility | Beneficial | Moderate |
| Lactobacillus reuteri DSM 17938 | Modulated Gut Microbiota Composition | Beneficial | Moderate |
| Lactobacillus reuteri DSM 17938 | Reduced Pro-Inflammatory Response | Beneficial | Moderate |