Lactobacillus rhamnosus induced epithelial cell apoptosis, ameliorates inflammation and prevents colon cancer development in an animal model.
- 2016-10
- Biomedicine & Pharmacotherapy 83
- Yaser Gamallat
- Abdo Meyiah
- E. Kuugbee
- A. Hago
- Gift Chiwala
- A. Awadasseid
- Djibril Bamba
- Xin Zhang
- X. Shang
- F. Luo
- Y. Xin
- PubMed: 27447122
- DOI: 10.1016/j.biopha.2016.07.001
Abstract
Background/aim: Probiotics have been suggested as prophylactic measure in colon carcinogenesis. This study aimed at determining the potential prophylactic activity of Lactobacillus rhamnosus GG CGMCC 1.2134 (LGG) strain on colorectal carcinogenesis via measuring its effect on Nuclear factor kappa B (NFκB) inflammatory pathway and apoptosis.
Materials and methods: 64 Sprague Dawley rats were grouped into four as follows; Group 1 (Healthy control), Group 2 (LGG), Group 3 (cancer control Dimethyl hydrazine (DMH)) and Group 4 (LGG+DMH). LGG was administered orally to LGG and LGG+DMH groups. Colon carcinogenesis was chemically induced in LGG+DMH and DMH groups by weekly injection of 40mg/kg DMH. Animals were sacrificed after 25 weeks of experiment and tumor characteristics assessed. The change in expression of NFκB-p65, COX-2, TNFα, Bcl-2, Bax, iNOS, VEGFα, β-catenin, Casp3 and p53 were evaluated by western blotting and qRT-PCR.
Results: LGG treatment significantly reduced tumor incidence, multiplicity and volume in LGG+DMH treatment group compared to DMH cancer control group. Also, LGG treatment reduced the expression of β-catenin and the inflammatory proteins NFκB-p65, COX-2 and TNFα; the anti-apoptotic protein Bcl-2, but increased the expression of the pro-apoptotic proteins Bax, casp3 and p53 compared with DMH group.
Conclusion: LGG have a potential protection effect against colon carcinogenesis; inducing apoptosis and ameliorating inflammation, and may hold a promise as bio-therapeutic dietary agent.
Keywords: Apoptosis; Colon cancer; Inflammation; Lactobacillus.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus rhamnosus CRL 1505 | Increased Cellular Apoptosis | Beneficial | Large |
| Lactobacillus rhamnosus CRL 1505 | Reduced Anti-apoptotic Protein Expression | Beneficial | Moderate |
| Lactobacillus rhamnosus CRL 1505 | Reduced Inflammatory Response | Beneficial | Large |
| Lactobacillus rhamnosus CRL 1505 | Reduced Tumor Incidence | Beneficial | Large |
| Lactobacillus rhamnosus PXN 54 | Increased Pro-Apoptotic Protein Expression | Beneficial | Moderate |
| Lactobacillus rhamnosus PXN 54 | Reduced Inflammation | Beneficial | Large |
| Lactobacillus rhamnosus PXN 54 | Reduced Inflammatory Protein Levels | Beneficial | Moderate |
| Lactobacillus rhamnosus UALr-06 | Reduced Expression of Beta-Catenin | Beneficial | Large |
| Lactobacillus rhamnosus UALr-06 | Reduced Tumor Incidence | Beneficial | Large |
| Lactobacillus rhamnosus UALr-06 | Reduced Tumor Multiplicity | Beneficial | Large |
| Lactobacillus rhamnosus UALr-06 | Reduced Tumor Volume | Beneficial | Large |
| Lactobacillus rhamnosus UALrh-18 | Increased Expression of Pro-apoptotic Proteins | Beneficial | Moderate |
| Lactobacillus rhamnosus UALrh-18 | Reduced Anti-apoptotic Protein Expression | Beneficial | Moderate |
| Lactobacillus rhamnosus UALrh-18 | Reduced Inflammatory Protein Expression | Beneficial | Moderate |