Lactobacillus rhamnosus LRa05 Ameliorate Hyperglycemia through a Regulating Glucagon-Mediated Signaling Pathway and Gut Microbiota in Type 2 Diabetic Mice.
- 2021-08-03
- Journal of Agricultural and Food Chemistry 69(31)
- Tao Wu
- Yongli Zhang
- W. Li
- Yunjiao Zhao
- Hairong Long
- Esther Mwizerwa Muhindo
- R. Liu
- Wenjie Sui
- Qian Li
- M. Zhang
- PubMed: 34340304
- DOI: 10.1021/acs.jafc.1c02925
Abstract
In this study, we aimed to explore the antidiabetic effects of Lactobacillus rhamnosus LRa05 on glucose metabolism and gut microbiota in type 2 diabetes mellitus (T2DM) mice. Our data indicated that the fasting blood glucose levels were reduced by 53.5% after treatment with LRa05 at a dose of 109 CFU·day-1. Meanwhile, LRa05 attenuated insulin resistance, relieved hepatic oxidative stress, and alleviated metabolic lipopolysaccharide-related inflammation in T2DM mice. LRa05 promoted the expression of glucose transporter 2, while it inhibited the expression of glucagon receptor, glucose-6-phosphatase, cellular adenosine-3'-5'-cyclic monophosphate-dependent protein kinase, and phosphoenolpyruvate carboxykinase in diabetic mice. Meanwhile, LRa05 reshaped gut microbiota, resulting in increased short-chain fatty acid bacteria (Alloprevotella and Bacteroides) and decreased proinflammatory bacteria (Odoribacter and Mucispirillum). Thus, LRa05 may be used as a functional food supplement for modulating the disorder glucose metabolism and gut microbiota in T2DM.
Keywords: Lactobacillus rhamnosus LRa05; T2DM; anti-hyperglycemic; glucagon signaling pathway; gut microbiota.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus rhamnosus CLR1505 | Altered Gut Microbiota | Beneficial | Moderate |
| Lactobacillus rhamnosus CLR1505 | Improved Insulin Resistance | Beneficial | Large |
| Lactobacillus rhamnosus CLR1505 | Reduced Hepatic Oxidative Stress | Beneficial | Moderate |
| Lactobacillus rhamnosus CLR1505 | Reduced Metabolic Inflammation | Beneficial | Moderate |
| Lactobacillus rhamnosus CLR1505 | Reduced Serum Glucose Levels | Beneficial | Large |
| Lactobacillus rhamnosus CRL 1505 | Altered Gut Microbiota | Beneficial | Moderate |
| Lactobacillus rhamnosus CRL 1505 | Improved Insulin Resistance | Beneficial | Large |
| Lactobacillus rhamnosus CRL 1505 | Reduced Hepatic Oxidative Stress | Beneficial | Moderate |
| Lactobacillus rhamnosus CRL 1505 | Reduced Metabolic Lipopolysaccharide-Related Inflammation | Beneficial | Moderate |
| Lactobacillus rhamnosus CRL 1505 | Reduced Serum Glucose Levels | Beneficial | Large |
| Lactobacillus rhamnosus LR04 | Altered Gut Microbiota | Beneficial | Moderate |
| Lactobacillus rhamnosus LR04 | Improved Hepatic Oxidative Stress | Beneficial | Moderate |
| Lactobacillus rhamnosus LR04 | Improved Insulin Resistance | Beneficial | Large |
| Lactobacillus rhamnosus LR04 | Reduced Serum Glucose Levels | Beneficial | Large |
| Lactobacillus rhamnosus LR05 | Improved Insulin Resistance | Beneficial | Large |
| Lactobacillus rhamnosus LR05 | Increased Short-Chain Fatty Acid-Producing Bacteria | Beneficial | Moderate |
| Lactobacillus rhamnosus LR05 | Reduced Hepatic Oxidative Stress | Beneficial | Moderate |
| Lactobacillus rhamnosus LR05 | Reduced Metabolic Inflammation | Beneficial | Moderate |
| Lactobacillus rhamnosus LR05 | Reduced Proinflammatory Bacteria | Beneficial | Moderate |
| Lactobacillus rhamnosus LR05 | Reduced Serum Glucose Levels | Beneficial | Large |
| Lactobacillus rhamnosus LR06 | Altered Gut Microbiota | Beneficial | Moderate |
| Lactobacillus rhamnosus LR06 | Improved Glucose Metabolism | Beneficial | Moderate |
| Lactobacillus rhamnosus LR06 | Improved Insulin Sensitivity | Beneficial | Moderate |
| Lactobacillus rhamnosus LR06 | Reduced Hepatic Oxidative Stress | Beneficial | Moderate |
| Lactobacillus rhamnosus LR06 | Reduced Serum Glucose Levels | Beneficial | Large |
| Lactobacillus rhamnosus LRa05 | Improved Insulin Sensitivity | Beneficial | Moderate |
| Lactobacillus rhamnosus LRa05 | Increased Beneficial Gut Microbiota | Beneficial | Moderate |
| Lactobacillus rhamnosus LRa05 | Reduced Hepatic Oxidative Stress | Beneficial | Moderate |
| Lactobacillus rhamnosus LRa05 | Reduced Proinflammatory Gut Bacteria | Beneficial | Moderate |
| Lactobacillus rhamnosus LRa05 | Reduced Serum Glucose Levels | Beneficial | Large |