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Abstract

Scope: This study aims to investigate the underlying mechanism of a specific probiotic strain on suppression of adipogenesis and inflammatory response in white adipose tissue (WAT) of high-fat diet (HFD)-fed mice.

Methods and results: Eight strains are screened in vitro for candidates of potential probiotics. Lactobacillus rhamnosus LRH05 (LRH05) and Lactobacillus reuteri LR47 (LR47) are screened out with lower triglyceride expression in vitro. The mice are fed a control diet (CD), HFD, or HFD supplemented with a dose of LRH05 or LR47 at 109 CFU per mouse per day for 10 weeks (n = 8), respectively. The results demonstrate that LRH05, but not LR47, significantly reduce body weight gain and the weight of WAT, as well as improve hepatic steatosis and glucose intolerance. LRH05 regulates the Mogat1, Igf-1, Mcp-1, and F4/80 mRNA expression and decreases macrophage infiltration in WAT. LRH05 shows an increase in butyric and propionic acid-producing bacteria, including Lachnoclostridium, Romboutsia, and Fusobacterium that is coincident with the increased fecal propionic acid and butyric acid levels.

Conclusion: LRH05 shows a strain-specific effect on ameliorating the pro-inflammatory process by reducing inflammatory macrophage infiltration and the expression of inflammation-related genes in mice. Thus, LRH05 can be considered a potential probiotic strain to prevent obesity.

Keywords: gut microbiota; inflammation; macrophages; obesity; probiotics.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus rhamnosus LR04Improved Glucose ToleranceBeneficial
Moderate
Lactobacillus rhamnosus LR04Improved Liver SteatosisBeneficial
Moderate
Lactobacillus rhamnosus LR04Reduced Body WeightBeneficial
Moderate
Lactobacillus rhamnosus LR04Reduced Inflammatory Gene ExpressionBeneficial
Large
Lactobacillus rhamnosus LR05Improved Glucose ToleranceBeneficial
Moderate
Lactobacillus rhamnosus LR05Improved Liver SteatosisBeneficial
Moderate
Lactobacillus rhamnosus LR05Reduced Body WeightBeneficial
Moderate
Lactobacillus rhamnosus LR05Reduced Macrophage Infiltration in Adipose TissueBeneficial
Large
Lactobacillus rhamnosus LRa05Improved Glucose ToleranceBeneficial
Moderate
Lactobacillus rhamnosus LRa05Improved Liver SteatosisBeneficial
Moderate
Lactobacillus rhamnosus LRa05Reduced Body WeightBeneficial
Moderate
Lactobacillus rhamnosus LRa05Reduced Inflammatory Gene ExpressionBeneficial
Large
Lactobacillus rhamnosus LRa05Reduced Macrophage Infiltration in Adipose TissueBeneficial
Large
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