Lactobacillus salivarius CML352 Isolated from Chinese Local Breed Chicken Modulates the Gut Microbiota and Improves Intestinal Health and Egg Quality in Late-Phase Laying Hens
- 2022-03-28
- Microorganisms 10(4)
- Chang Xu
- Fuxiao Wei
- Xinyue Yang
- Yuqing Feng
- Dan Liu
- Yongfei Hu
- PubMed: 35456777
- DOI: 10.3390/microorganisms10040726
Abstract
Lactobacillus strains with fine probiotic properties are continuously needed in the laying hen industry to improve the animals' gut health and production performance. In this study, we isolated 57 Lactobacillus strains from the gut microbiota of 17 different chicken breeds in China. We characterized the probiotic features of these isolates, and evaluated the effects of a selected strain, Lactobacillus salivarius CML352, on the production performance and gut health of the late-phase laying hens. The results showed that the isolates varied much in probiotic properties, among which L. salivarius CML352 displayed high acid and bile salt tolerance, high hydrophobicity, auto-aggregation, and antibacterial activities. Whole genome sequencing analysis showed that CML352 was closely related to a strain isolated from human fecal samples, but had different functional potentials. Dietary supplementary of L. salivarius CML352 significantly reduced the Firmicutes to Bacteroidetes ratio, increased the expression of Muc-2, and decreased the expression of MyD88, IFN-γ, and TLR-4. Furthermore, strain CML352 reduced the birds' abdominal fat deposition, and improved egg quality. Taken together, this study indicated that the newly isolated L. salivarius strain might be a worthy probiotic with positive impacts on the intestinal health and production performance of late-phase laying hens.
Keywords: Lactobacillus salivarius; egg quality; gut microbiota; intestinal health; laying hens.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus Salivarius | Improved Egg Quality | Beneficial | Moderate |
| Lactobacillus Salivarius | Increased MUC-2 Expression | Beneficial | Moderate |
| Lactobacillus Salivarius | Reduced Firmicutes/Bacteroidetes Ratio | Beneficial | Moderate |
| Lactobacillus Salivarius | Reduced Visceral Fat | Beneficial | Moderate |
| Lactobacillus salivarius SD-5208 | Improved Egg Quality | Beneficial | Moderate |
| Lactobacillus salivarius SD-5208 | Improved Intestinal Function | Beneficial | Moderate |
| Lactobacillus salivarius SD-5208 | Increased MUC-2 Expression | Beneficial | Moderate |
| Lactobacillus salivarius SD-5208 | Reduced Firmicutes/Bacteroidetes Ratio | Beneficial | Moderate |
| Lactobacillus salivarius SD-5208 | Reduced IFN-γ Expression | Beneficial | Moderate |
| Lactobacillus salivarius SD-5208 | Reduced MyD88 Expression | Beneficial | Moderate |
| Lactobacillus salivarius SD-5208 | Reduced TLR4 Expression | Beneficial | Moderate |
| Lactobacillus salivarius SD-5208 | Reduced Visceral Fat | Beneficial | Moderate |
| Lactobacillus salivarius SD-5851 | Improved Egg Quality | Beneficial | Moderate |
| Lactobacillus salivarius SD-5851 | Improved Intestinal Function | Beneficial | Moderate |
| Lactobacillus salivarius SD-5851 | Reduced Visceral Fat | Beneficial | Moderate |
| Lactobacillus salivarius UALb-07 | Improved Egg Quality | Beneficial | Moderate |
| Lactobacillus salivarius UALb-07 | Increased MUC-2 Expression | Beneficial | Moderate |
| Lactobacillus salivarius UALb-07 | Reduced Firmicutes/Bacteroidetes Ratio | Beneficial | Moderate |
| Lactobacillus salivarius UALb-07 | Reduced IFN-γ Expression | Beneficial | Moderate |
| Lactobacillus salivarius UALb-07 | Reduced MyD88 Expression | Beneficial | Moderate |
| Lactobacillus salivarius UALb-07 | Reduced TLR4 Expression | Beneficial | Moderate |
| Lactobacillus salivarius UALb-07 | Reduced Visceral Fat | Beneficial | Moderate |
| Lactobacillus salivarius UALs07 | Improved Egg Quality | Beneficial | Moderate |
| Lactobacillus salivarius UALs07 | Increased MUC-2 Expression | Beneficial | Moderate |
| Lactobacillus salivarius UALs07 | Reduced Firmicutes/Bacteroidetes Ratio | Beneficial | Moderate |
| Lactobacillus salivarius UALs07 | Reduced IFN-γ Expression | Beneficial | Moderate |
| Lactobacillus salivarius UALs07 | Reduced MyD88 Expression | Beneficial | Moderate |
| Lactobacillus salivarius UALs07 | Reduced TLR4 Expression | Beneficial | Moderate |
| Lactobacillus salivarius UALs07 | Reduced Visceral Fat | Beneficial | Moderate |
| Lactobacillus salivarius UALs07 UAS | Improved Egg Quality | Beneficial | Moderate |
| Lactobacillus salivarius UALs07 UAS | Improved Gastrointestinal Health | Beneficial | Large |
| Lactobacillus salivarius UALs07 UAS | Reduced Visceral Fat | Beneficial | Moderate |
| Lactobacillus salivarius VPro 15 | Improved Intestinal Mucosal Immunity | Beneficial | Moderate |