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Lactobacillus salivarius SNK-6 Regulates Liver Lipid Metabolism Partly via the miR-130a-5p/MBOAT2 Pathway in a NAFLD Model of Laying Hens

  • 2022-12-19
  • Cells 11(24)
    • Lihui Zhu
    • R. Liao
    • Jiwen Huang
    • Changfeng Xiao
    • Yunzhou Yang
    • Huiying Wang
    • D. He
    • Huaxiang Yan
    • Changsuo Yang

Abstract

Lactobacillus spp., as probiotics, have shown efficacy in alleviating nonalcoholic fatty liver disease (NAFLD). Here, we screened a new probiotic strain, Lactobacillus salivarius SNK-6 (L. salivarius SNK-6), which was isolated from the ileum of healthy Xinyang black-feather laying hens in China. We investigated the beneficial activity of L. salivarius SNK-6 in a NAFLD model in laying hens and found that L. salivarius SNK-6 inhibited liver fat deposition and decreased serum triglyceride levels and activity of aspartate transaminase and alanine transaminase. MBOAT2 (membrane-bound O-acyltransferase domain containing 2) was directly targeted by miR-130a-5p, which was downregulated in the liver of NAFLD laying hens but reversed after L. salivarius SNK-6 treatment. Downregulation of MBOAT2, L. salivarius SNK-6 supplementation in vivo, and L. salivarius SNK-6 cell culture treatment in vitro suppressed the mRNA expression of genes involved in the PPAR/SREBP pathway. In addition, 250 metabolites were identified in the supernatants of L. salivarius SNK-6 culture media, and most of them participated in metabolic pathways, including amino acid, carbohydrate, and lipid metabolism. Targeted metabolomic analysis revealed that acetate, butyrate, and propionate were the most abundant short-chain fatty acids, while cholic acid, ursodeoxycholic acid, chenodeoxycholic acid, and tauroursodeoxycholic acid were the four most-enriched bile acids among L. salivarius SNK-6 metabolites. This may have contributed to the reparative effect of L. salivarius SNK-6 in the NAFLD chicken model. Our study suggested that L. salivarius SNK-6 alleviated liver damage partly via the miR-130a-5p/MBOAT2 signaling pathway.

Keywords: Lactobacillus salivarius SNK-6; PPAR/SREBP pathway; fat deposition; fatty liver.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus SalivariusDecreased MBOAT2 ExpressionBeneficial
Moderate
Lactobacillus SalivariusReduced Alanine Transaminase ActivityBeneficial
Moderate
Lactobacillus SalivariusReduced Aspartate Transaminase ActivityBeneficial
Moderate
Lactobacillus SalivariusReduced Liver Fat DepositionBeneficial
Moderate
Lactobacillus SalivariusReduced Triglyceride LevelsBeneficial
Moderate
Lactobacillus salivarius HA-118Improved Liver FunctionBeneficial
Large
Lactobacillus salivarius HA-118Reduced Liver Enzyme ActivityBeneficial
Moderate
Lactobacillus salivarius HA-118Reduced Liver FatBeneficial
Large
Lactobacillus salivarius HA-118Reduced Triglyceride LevelsBeneficial
Moderate
Lactobacillus salivarius HA-118Regulated Liver Lipid MetabolismBeneficial
Moderate
Lactobacillus salivarius HA-118Suppressed PPAR/SREBP Pathway Gene ActivityBeneficial
Moderate
Lactobacillus salivarius SD-5208Reduced Alanine Transaminase ActivityBeneficial
Moderate
Lactobacillus salivarius SD-5208Reduced Aspartate Transaminase ActivityBeneficial
Moderate
Lactobacillus salivarius SD-5208Reduced Liver Fat DepositionBeneficial
Moderate
Lactobacillus salivarius SD-5208Regulated miR-130a-5p LevelsBeneficial
Moderate
Lactobacillus salivarius SD-5208Suppressed PPAR/SREBP Pathway Gene ExpressionBeneficial
Moderate
Lactobacillus salivarius SD-5851Decreased MBOAT2 ExpressionBeneficial
Moderate
Lactobacillus salivarius SD-5851Reduced Liver Fat DepositionBeneficial
Moderate
Lactobacillus salivarius SD-5851Reduced Triglyceride LevelsBeneficial
Moderate
Lactobacillus salivarius SD-5851Suppressed PPAR/SREBP Pathway Gene ExpressionBeneficial
Moderate
Lactobacillus salivarius UALs07Modulated PPAR/SREBP PathwayBeneficial
Moderate
Lactobacillus salivarius UALs07Reduced Alanine Transaminase ActivityBeneficial
Moderate
Lactobacillus salivarius UALs07Reduced Aspartate Transaminase ActivityBeneficial
Moderate
Lactobacillus salivarius UALs07Reduced Liver Fat DepositionBeneficial
Moderate
Lactobacillus salivarius UALs07Reduced Triglyceride LevelsBeneficial
Moderate
Lactobacillus salivarius UALs07Regulation of miR-130a-5p ExpressionBeneficial
Moderate
Lactobacillus salivarius UALs07 UASReduced Alanine Transaminase ActivityBeneficial
Moderate
Lactobacillus salivarius UALs07 UASReduced Aspartate Transaminase ActivityBeneficial
Moderate
Lactobacillus salivarius UALs07 UASReduced Liver Fat DepositionBeneficial
Moderate
Lactobacillus salivarius UALs07 UASRegulation of miR-130a-5p ExpressionBeneficial
Moderate
Lactobacillus salivarius UALs07 UASSuppressed PPAR/SREBP Pathway Gene ExpressionBeneficial
Moderate
Lactobacillus salivarius VPro 15Regulated Lipid MetabolismBeneficial
Moderate
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