Lactobacillus salivarius SNK-6 Regulates Liver Lipid Metabolism Partly via the miR-130a-5p/MBOAT2 Pathway in a NAFLD Model of Laying Hens
- 2022-12-19
- Cells 11(24)
- Lihui Zhu
- R. Liao
- Jiwen Huang
- Changfeng Xiao
- Yunzhou Yang
- Huiying Wang
- D. He
- Huaxiang Yan
- Changsuo Yang
- PubMed: 36552896
- DOI: 10.3390/cells11244133
Abstract
Lactobacillus spp., as probiotics, have shown efficacy in alleviating nonalcoholic fatty liver disease (NAFLD). Here, we screened a new probiotic strain, Lactobacillus salivarius SNK-6 (L. salivarius SNK-6), which was isolated from the ileum of healthy Xinyang black-feather laying hens in China. We investigated the beneficial activity of L. salivarius SNK-6 in a NAFLD model in laying hens and found that L. salivarius SNK-6 inhibited liver fat deposition and decreased serum triglyceride levels and activity of aspartate transaminase and alanine transaminase. MBOAT2 (membrane-bound O-acyltransferase domain containing 2) was directly targeted by miR-130a-5p, which was downregulated in the liver of NAFLD laying hens but reversed after L. salivarius SNK-6 treatment. Downregulation of MBOAT2, L. salivarius SNK-6 supplementation in vivo, and L. salivarius SNK-6 cell culture treatment in vitro suppressed the mRNA expression of genes involved in the PPAR/SREBP pathway. In addition, 250 metabolites were identified in the supernatants of L. salivarius SNK-6 culture media, and most of them participated in metabolic pathways, including amino acid, carbohydrate, and lipid metabolism. Targeted metabolomic analysis revealed that acetate, butyrate, and propionate were the most abundant short-chain fatty acids, while cholic acid, ursodeoxycholic acid, chenodeoxycholic acid, and tauroursodeoxycholic acid were the four most-enriched bile acids among L. salivarius SNK-6 metabolites. This may have contributed to the reparative effect of L. salivarius SNK-6 in the NAFLD chicken model. Our study suggested that L. salivarius SNK-6 alleviated liver damage partly via the miR-130a-5p/MBOAT2 signaling pathway.
Keywords: Lactobacillus salivarius SNK-6; PPAR/SREBP pathway; fat deposition; fatty liver.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus Salivarius | Decreased MBOAT2 Expression | Beneficial | Moderate |
| Lactobacillus Salivarius | Reduced Alanine Transaminase Activity | Beneficial | Moderate |
| Lactobacillus Salivarius | Reduced Aspartate Transaminase Activity | Beneficial | Moderate |
| Lactobacillus Salivarius | Reduced Liver Fat Deposition | Beneficial | Moderate |
| Lactobacillus Salivarius | Reduced Triglyceride Levels | Beneficial | Moderate |
| Lactobacillus salivarius HA-118 | Improved Liver Function | Beneficial | Large |
| Lactobacillus salivarius HA-118 | Reduced Liver Enzyme Activity | Beneficial | Moderate |
| Lactobacillus salivarius HA-118 | Reduced Liver Fat | Beneficial | Large |
| Lactobacillus salivarius HA-118 | Reduced Triglyceride Levels | Beneficial | Moderate |
| Lactobacillus salivarius HA-118 | Regulated Liver Lipid Metabolism | Beneficial | Moderate |
| Lactobacillus salivarius HA-118 | Suppressed PPAR/SREBP Pathway Gene Activity | Beneficial | Moderate |
| Lactobacillus salivarius SD-5208 | Reduced Alanine Transaminase Activity | Beneficial | Moderate |
| Lactobacillus salivarius SD-5208 | Reduced Aspartate Transaminase Activity | Beneficial | Moderate |
| Lactobacillus salivarius SD-5208 | Reduced Liver Fat Deposition | Beneficial | Moderate |
| Lactobacillus salivarius SD-5208 | Regulated miR-130a-5p Levels | Beneficial | Moderate |
| Lactobacillus salivarius SD-5208 | Suppressed PPAR/SREBP Pathway Gene Expression | Beneficial | Moderate |
| Lactobacillus salivarius SD-5851 | Decreased MBOAT2 Expression | Beneficial | Moderate |
| Lactobacillus salivarius SD-5851 | Reduced Liver Fat Deposition | Beneficial | Moderate |
| Lactobacillus salivarius SD-5851 | Reduced Triglyceride Levels | Beneficial | Moderate |
| Lactobacillus salivarius SD-5851 | Suppressed PPAR/SREBP Pathway Gene Expression | Beneficial | Moderate |
| Lactobacillus salivarius UALs07 | Modulated PPAR/SREBP Pathway | Beneficial | Moderate |
| Lactobacillus salivarius UALs07 | Reduced Alanine Transaminase Activity | Beneficial | Moderate |
| Lactobacillus salivarius UALs07 | Reduced Aspartate Transaminase Activity | Beneficial | Moderate |
| Lactobacillus salivarius UALs07 | Reduced Liver Fat Deposition | Beneficial | Moderate |
| Lactobacillus salivarius UALs07 | Reduced Triglyceride Levels | Beneficial | Moderate |
| Lactobacillus salivarius UALs07 | Regulation of miR-130a-5p Expression | Beneficial | Moderate |
| Lactobacillus salivarius UALs07 UAS | Reduced Alanine Transaminase Activity | Beneficial | Moderate |
| Lactobacillus salivarius UALs07 UAS | Reduced Aspartate Transaminase Activity | Beneficial | Moderate |
| Lactobacillus salivarius UALs07 UAS | Reduced Liver Fat Deposition | Beneficial | Moderate |
| Lactobacillus salivarius UALs07 UAS | Regulation of miR-130a-5p Expression | Beneficial | Moderate |
| Lactobacillus salivarius UALs07 UAS | Suppressed PPAR/SREBP Pathway Gene Expression | Beneficial | Moderate |
| Lactobacillus salivarius VPro 15 | Regulated Lipid Metabolism | Beneficial | Moderate |