Matrix effect of four Chinese medicinal herbs on colloidal gold immunoassay for organophosphorus pesticides.
- 2026-01-01
- Open life sciences 21(1)
- Maoqiong Wei
- Ziri Wang
- Li Wang
- Shanshan Lan
- Zhenhuan Liu
- Xin Lin
- Hongcheng Liu
- PubMed: 41726548
- DOI: 10.1515/biol-2025-1201
Study Design
- Methods
- quantitative analyses using gold immunochromatographic assay (GICA) for chlorpyrifos, triazophos, and isocarbophos residues in four Chinese medicinal herbs
- Funding
- Unclear
The matrix effect (ME) presents a serious problem for the immunoassay of pesticide residues, considerably limiting its practical application in Chinese medicinal herbs and other food products. The objective of this study was to achieve reduced matrix effect and improved detection accuracy of colloidal gold test strips. For that reason, four types of Chinese medicinal herbs (Ophiopogon japonicus, Bulbus Fritillaria, Polygonatum sibiricum, and Folium Artemisiae Argyi) were used as samples to investigate the effect of the Chinese medicinal herb matrix on the gold immunochromatographic assay (GICA) for analyzing three organophosphorus pesticide (chlorpyrifos, triazophos, and isocarbophos) residues. In the quantitative analyses of chlorpyrifos, triazophos and isoprocarbophos, the extraction method completely eliminated the matrix interference, and the sensitivities of GICA were increased by about 8.3, 1.7 and 1 times, respectively, compared with those of the standard operating instructions for test strips. The mechanism of the matrix of Chinese herbal medicines on the ME of colloidal gold test strips was preliminarily explored. It is reasonable to hypothesize that natural products and secondary metabolites have a significant effect on GICA, leading to ME.