Modulation of Behavioral, Biochemical, Immunomodulatory, and Transcriptional Profiles by the Strain Limosilactobacillus fermentum U-21 in Combined Model of Parkinson's Disease in Wistar Rats.

2025-12-31
International journal of molecular sciences 27(1)

PubMed: 41516320
DOI: 10.3390/ijms27010446

Study Design

Population: Wistar rats
Methods: A combined LPS- and lactacystin (LAC)-induced Parkinson's disease model was established in Wistar rats; LfU21 administration effects were assessed using behavioral, biochemical, immunomodulatory, and transcriptomic biomarkers.

Rigorous Journal
Animal Study

Since there is currently no cure for Parkinson's disease, pharmacobiotic approaches based on gut microbiota-capable of producing pharmacologically active compounds-are under development. In this study, we propose LfU21, derived from the strain Limosilactobacillus fermentum U-21, as a candidate pharmacobiotic. To evaluate its efficacy, a combined LPS- and lactacystin (LAC)-induced Parkinson's disease model was established in Wistar rats. Effects were assessed using behavioral, biochemical, immunomodulatory, and transcriptomic biomarkers. LfU21 administration reduced α-synuclein levels, altered motor performance in the "Rung ladder" test, and modulated bdnf gene expression in the right and left striata. Under LPS exposure, LfU21 prevented alterations in immune response markers, GSH levels, drd2 and bdnf gene expression, and intestinal goblet cell counts. In LAC and LAC + LPS groups, LfU21 mitigated the rise in α-synuclein, the decline in bdnf expression, and behavioral deficits in the "Open Field" and "Rung ladder" tests, respectively. The multifunctional activity of LfU21 in a combined Parkinson's disease model underscores its therapeutic potential and helps identify a target patient cohort for future clinical trials.

Research Insights

Supplement	Dose	Health Outcome	Effect Type	Effect Size	Source
Lactobacillus rhamnosus	—	Reduced Behavioral Deficits in Parkinson's Disease Model	Beneficial	Moderate	View source In LAC and LAC + LPS groups, LfU21 mitigated the rise in α-synuclein, the decline in bdnf expression, and behavioral deficits in the "Open Field" and "Rung ladder" tests, respectively.