Molecular Detection and Identification of Plant-Associated Lactiplantibacillus plantarum

Abstract

Lactiplantibacillus plantarum is a lactic acid bacterium often isolated from a wide variety of niches. Its ubiquity can be explained by a large, flexible genome that helps it adapt to different habitats. The consequence of this is great strain diversity, which may make their identification difficult. Accordingly, this review provides an overview of molecular techniques, both culture-dependent, and culture-independent, currently used to detect and identify L. plantarum. Some of the techniques described can also be applied to the analysis of other lactic acid bacteria.

Keywords: 16S rRNA; WGS; genotyping; housekeeping genes; qPCR; shotgun.