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Optimization for production of exopolysaccharides with antitumor activity in vitro from Paecilomyces hepiali.

  • 2014-01
  • Carbohydrate Polymers 99
    • Zhongwei Wu
    • Junwen Lu
    • Xiaoqing Wang
    • Bing Hu
    • Hong Ye
    • Jia-Qin Fan
    • Muhammad Abid
    • Xiaoxiong Zeng


In the present study, optimal medium for the growth of mycelia and the production of exopolysaccharides from Paecilomyces hepiali HN1 (PHEPS) in submerged culture was investigated. As a result, the maximum production of mycelia (12.98 ± 0.14 g/L) and PHEPS (5.33 ± 0.11 g/L) were achieved under the optimal medium of sucrose 46.08 g/L, yeast extract 4.71 g/L, (NH₄)₂SO₄ 5.72 g/L, KH₂PO₄ 1.70 g/L, CaCl₂ 0.50 g/L, MgSO₄ 0.50 g/L, potato extract 1% and malt extract 1%. Furthermore, the antitumor activity of PHEPS in vitro was evaluated by using three cell lines of human liver tumor HepG2 cells, breast cancer MCF-7 cells and cervical cancer Hela cells. It was found that PHEPS exhibited relative higher anti-proliferative activity against HepG2 cells than MCF-7 cells and Hela cells. At a concentration of 500 μg/mL and 72 h treatment, the inhibition rate of PHEPS on HepG2 cells reached to 62.58%. All these results suggested that PHEPS could be explored as novel natural antitumor agent with great potential application.

Keywords: Antitumor activity; Optimization; Paecilomyces hepiali HN1; Polysaccharides.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Paecilomyces hepialiIncreased Anticancer ActivityBeneficial
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